Method: ,In order to determine the minimum inhibitory concentration (MIC) values for nanosilver (0.1 mg/ml) and CHX (20 mg/ml), two series of serial dilutions, within the range of 1:2 to 1:1024, were made for each. The culture media were Trypticase Soy Broth and Brain Heart Infusion broth for chlorhexidine and nanosilver respectively. Standard microbial suspensions were then added to tubes containing the diluted samples. After a 24-hour incubation period at 37˚c, small amounts of the tube contents were inoculated onto blood agar media and plates were incubated for another 24 hours. Finally, MIC values were determined and data were compiled in a table.
Result: ,The results revealed that nanosilver at 1/50 and 1/200 the concentrations of chlorhexidine was equally effective against E. faecalis and C. albicans, respectively.
Conclusion: ,It was concluded that nanosilver- based suspension has the potential of being used as antimicrobial agent in endodontic treatments; however, further studies are needed to examine other main characteristics of this irrigant.