Method: Low-speed burs and Er,Cr:YSGG laser devices were used for cavity preparations and Mineral Trioxide Aggregate (MTA Angelus), Biodentine (Septodont), Amalgam (Cavex Avalloy), Composite resin (3M ESPE) and dentin bonding system (Kerr, Optibond All-In-One) as root-end filling materials. Sixty-four extracted single rooted human teeth were decoronated to a length of 15 mm and the canals were prepared using ProTaper system with a crown-down technique and filled by lateral condensation technique. Both 3-mm of apical resection and root-end cavity preparation were performed by Low-speed burs or Er,Cr:YSGG laser. The roots were randomly divided into eight experimental groups (n=8 per group) as Group 1: Low-speed carbide bur + MTA-Angelus; Group 2: Low-speed bur + Biodentine; Group 3: Low-speed bur + Composite resin; Group 4: Low-speed bur + Amalgam; Group 5: Er,Cr:YSGG laser + MTA-Angelus; Group 6: Er,Cr:YSGG laser + Biodentine; Group 7: Er,Cr:YSGG laser + Composite resin; Group 8: Er,Cr:YSGG laser + Amalgam. After filling the root-end cavities, the root surfaces were coated with a double layer of nail varnish except the retrograde-filled surfaces and roots were immersed in Indian ink for 15 days. Dye penetration level was assessed with clearing technique. Data were statistically analyzed by using Kruskal-Wallis and Mann Whitney U tests.
Result: There was no significant difference between the root-end cavity preparation techniques (p>0.05). However; there were statistical significant differences between amalgam groups and Biodentine and MTA-Angelus groups (p <0.05). Amalgam groups showed significantly the lowest sealing ability when compared with other test groups (p <0.05).
Conclusion: Within the limitations of this study, Biodentine and MTA-angelus performed higher sealing ability as a root end-filling material.