Variety of resins has been introduced into dental practice for the manufacturing of dental prostheses and their efficacy has been based on physical, chemical and biological properties. Recently, 3D printing techniques have been introduced to manufacture new denture base materials. The aim of this study was to evaluate genotoxicity of new 3D printing denture material Dentca (Dentca, Inc.).
Method:
Raw Dentca material was mixed according to the manufacturer instructions. Thereafter, material specimens were generated through DLP-3D printer (Carima, Inc.). The printed specimens were washed with isopropanol alcohol, dried, post-cured under UV/Vis curing unit (Honle UV America Inc.) for 1h and cut into 1x1x0.2cm3 size. Samples (16 totals) were divided in two groups: polished (8) and unpolished (8). Samples were storage in artificial saliva for 1 day and placed in prepared lymphocyte cell culture. Alkaline comet assay test was used for genotoxicity assessment using epifluorescent microscope Olympus BX 51 (Olympus, Japan) connected to Comet Assay IV analysis system (Perceptive Instruments, UK). Results are expressed using tail length (µm) and tail intensity (% of DNA in comet tail) and presented as mean and median and S.D. of two scorings. The same procedure was repeated after 7 and 14 days. One-way ANOVA with Tukey post hoc analysis by using Statistica 10 (StatSoft, OK, USA) was used for statistical analysis (p≤0.05).
Result:
In both cases Dentca did not show statistical significant difference when compared to negative control group (redistilled H2O)(p<0.01). The highest tail length values are recorded for both, polished and unpolished samples after 7 days while the highest values for tail intensity were recorded for both groups after 14 days.
Conclusion: The new 3D printing denture material Dentca show excellent biocompatibility when compared to negative control and according to the results is safe for clinical use.