IADR Abstract Archives
Biocompatibility of Nanohydroxyapatites in 2- and 3-Dimensional Culture Systems
Methods: Commercially obtained primary MSCs and primary osteoblasts (HOBs), and the osteoblastic cell lines MG63 and Saos-2 were used. For 2D-cultures, 5x104 cells were incubated as monolayers for 48h with nHA pastes. For 3D-cultures 2x106 cells were seeded onto polyglycolic acid discs before incubation with nHA pastes. Cell viability was assessed using PrestoBlue and Cellstain was used to visualise live and dead cells. Cellular uptake of nanoparticles of HA was investigated using transmission electron microscopy (TEM).
Results: All nHAs showed some toxicity in 2D-cell cultures. MSCs and the more mature osteoblastic cells (HOBs and Saos-2) were the most sensitive showing, respectively, 69%, 87% and 65% reduced cell viability to BP1 while the more immature MG63’s were less sensitive (39% reduction). TEM showed numerous HA-nanoparticles in the cell cytoplasm which may have resulted in the observed toxicities. 3D-cultures of MG63 and MSCs showed less toxicity (10-30%) to the nHAs than 2D-cultures and may be more reflective of reported in vivo data.
Conclusions: 3D-culture systems may be more useful than 2D-culture systems for testing biocompatibility of nHA’s as they were more reflective of nHA in vivo.
Acknowledgemenst: The research leading to these results has received funding from the EU FP7 Programme under grant agreement n° 315679.