Methods: We used 3T3 fibroblast cells exposed to different dilutions of medium previously incubated for 24 hours with the three different cements that were prepared according to the manufacturer’s instructions. The resin cement was prepared in two ways: light cured and non-light cured. After 24 hours cell viability and genotoxicity were measured by cristal violet and micronuclei assays, respectively.
Results: Results were the same as MTT assay, showing a decrease of cell viability and functional activity of mitochondria in all cements, which is directly proportional to the amount of extract added. A decrease in viability is most pronounced for the resin cement, especially when it is not cured. We also present the results obtained for chromosomal integrity indicating genotoxicity of these cements.
Conclusion: Comparing to resin cements the cell viability is highest in presence of glass ionomer. Light cure of resin cements is important to decrease fibroblastic cytotoxicity. Maximum cytotoxicity was observed in the presence of resin cements, correlating positively with the amount of monomers released (based on the literature). Maybe there are advantages by using absolute isolation techniques, particularly with resin cements.