Effectiveness Of Myostatin Knockdown On Jaw Muscle Activity
Objective: Myostatin, a member of TGF-β superfamily, is known as a negative regulator of cell growth and differentiation in skeletal muscle. RNA interference (RNAi) offers a novel therapeutic strategy by efficiently silencing target genes known to promote disease. This study evaluated the effectiveness of local administration of cationic liposome-delivered myostatin-targeting siRNA (Mst-siRNA-lipoplexes) on jaw muscle activity.
Methods: Mst-siRNA and scrambled (scr)-siRNA-lipoplexes were injected into the masseter muscles of dystrophin-deficient mdx mice, which model Duchenne muscular dystrophy. One week after injection, the masseter muscles were dissected for histometric analysis. To evaluate changes in masseter muscle activity, electromyographic (EMG) measurements were performed.
Results: Sectional area of masseter muscle fibers treated with Mst-siRNA-lipoplexes was significantly larger compared to the control injected with scr-siRNA-lipoplexes. The daily integral value of masseter muscle activity was significantly increased by knockdown of myostatin.
Conclusion: These data suggest that local administration of Mst-siRNA-lipoplexes may be a useful tool for treating muscle atrophy diseases including muscular dystrophy.
Division: Pan European Region Meeting
Meeting:2014 Pan European Region Meeting (Dubrovnik, Croatia) Location: Dubrovnik, Croatia
Year: 2014 Final Presentation ID:456 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Mori, Hiroyo
( Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, , Japan
)
Kawai, Nobuhiko
( Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, , Japan
)
Bayarsaikhan, Od
( Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, , Japan
)
Hichijo, Natsuko
( Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, , Japan
)
Kinouchi, Nao
( Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, , Japan
)
Ishida, Tatsuhiro
( Department of Pharmacokinetics and Biopharmaceutics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, , Japan
)
Noji, Sumihare
( Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima, , Japan
)
Tanaka, Eiji
( Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, , Japan
)