Effect Of Dentin On The Antimicrobial Properties Of Hyperpure Chlorine-Dioxide
Objective: Previously we found that the high purity chlorine-dioxide (ClO2) has a very potent disinfectant efficacy on oral pathogenic microorganisms and as a root canal irrigant it is able to eliminate the experimental Enterococcus faecalis infection from the root canal system. This study examines whether the presence of dentin powder influences the antibacterial efficacy of ClO2. Methods: In an in vitro dentin powder model the following irrigants were tested against planktonic Enterococcus faecalis: 2% chlorhexidine (CHX), 2.5% sodium hypochlorite (NaOCl), 0.12% ClO2 (Solumium) and one local root canal medicament: saturated Ca(OH)2. Survival of bacteria exposed to agents without and with human dentin powder or preincubated with dentin powder was investigated. The effect of the dentin powder on ClO2 concentration was investigated by titrations. Results: Without dentin ClO2 completely killed all Enterococcus faecalis already after 1 minute and Ca(OH)2 after 60 minutes. In case of CHX and NaOCl small amount of bacteria survived in all investigated periods. The presence of dentin powder decreased the concentration of ClO2 and attenuated the antibacterial efficiency of both ClO2 and Ca(OH)2, but did not decrease of CHX and NaOCl. Preincubation with dentin powder caused significant loss of antibacterial activity of all investigated agents, ClO2 and Ca(OH)2 having the highest reduction. Conclusion: The practical validity of the in vitro disinfecting efficacy measurements without dentin powder has to be confirmed in the presence of dentin powder. As the presence of dentin powder had a negative effect on the efficacy of disinfectants, the importance of elimination of dentin scrapings and smear layer from the root canal system during endodontic treatments is highly recommended. There is a need to seek a better intertreatment, intracanal medicament than Ca(OH)2. Hyperpure ClO2 can be an effective final rinse in endodontics or a powerful cavity cleaner in conservative dentistry. Support: Semmelweis University FOK/DH/2013.
Division: Pan European Region Meeting
Meeting:2014 Pan European Region Meeting (Dubrovnik, Croatia) Location: Dubrovnik, Croatia
Year: 2014 Final Presentation ID:455 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Lohinai, Zsolt M.
( Semmelweis University, Budapest, , Hungary
; MinDent 1987 Company, Budapest, , Hungary
)
Gyurkovics, Milán
( Semmelweis University, Budapest, , Hungary
)
Ghidan, Agoston
( Semmelweis University, Budapest, , Hungary
)
Megyesi, Marianna
( Budapest University of Technology and Economics, Budapest, , Hungary
)
Herczegh, Anna
( Semmelweis University, Budapest, , Hungary
)