Oral cavity microbiocenosis in children with intellectual disabilities

Method:  A total of 220 children (aged between 12 and 15) with intellectual disabilities were examined by using WHO oral health assessment form. Caries experience was estimated using a sum of Decayed (D), Missing (M), Filled (F) teeth and presented as means 95% Cls. Oral hygiene status (Oral hygiene index simplified, Green, Vermillion, 1964) and salivary levels of Str. mutans (by express method such as «Dentocult LB» , Liechtenstein). Cultures taken from toothbrushes  to find aerobic and anaerobic flora by using a set of plates manufactured by «PLIVA-Lachema»(Czech Republic) on the basis of microbiological analyzer iEMS Reader MF (Thermo Fisher Scien. Inc., Finland) and «Microb-2» software (Russia). Dichotomous and continuous data were analysed using χ² and Mann-Whitney tests.

Result: The results of studies show that the concentrations of Streptococci and Staphilococcus epidermidis provid 7,0 lg CFU/ml and 6,0 lg CFU/ml respectively in 100 percent of children. Results of the study revealed a high concentration of pathogenic organisms which are normally non-existent in a healthy person. Concentration of Staphilococcus aureus was 2,0 lg CFU/ml in 26,6%, that of Pseudomonas aerugenosa was 4,0 lg CFU/ml in 2 percent of the children, ε.coli was 2,0 lg CFU/mi in 55,5% including pathogenic Enterococcus faecium in 8% of the cases. Almost complete absence of Lactobacilli and growth of Candida alb. correspond to the III – IV degree of clinical manifestations of dysbiosis.

Conclusion:

The findings of quantitative and qualitative composition of the microbial flora suggest the need to find effective methods of prevention and treatment of dental diseases for this social group.