IADR Abstract Archives
Effect of Buffering Solutions on Resins Composites
Objectives: Interaction of composites and saliva in the complex oral environment results in surface and structural composite alterations. The aim of this study was to analyze the monomer elution of composites after storage in different buffered solutions, resembling saliva and oral conditions.
Methods: A UDMA- and BisGMA-containing nanohybrid composite resin was used (Evetric, Ivoclar Vivadent, Schaan, Liechtenstein). Four groups (n=10) were prepared for each storage solution: phosphate buffering solution, distilled water, artificial saliva and bicarbonate buffering solution. Teflon molds (diameter 10mm, thickness 2mm) were used for samples fabrication. Samples were cured using a LED light-curing unit (Bluephase, Ivoclar Vivadent, Schaan, Liechtenstein) and were stored in the tested solutions at 37o C in the dark. After 15 days of storage, 20 μl of the storage solution was removed and High Performance Liquid Chromatography (HPLC) was processed to analyze the elution of leached monomers. Data were analyzed with respect to the storage solution. The average amount of monomer elution in ppm and standard deviation were calculated for each storage solution. Statistical analysis was performed at level of significance p=0.05.
Results: No Bis-GMA and UDMA monomers were detected in the tested solutions after 15 days storage. However, Bisphenol A (BPA) was detected in phosphate and bicarbonate buffering solutions and distilled water.
Conclusion: Leaching of monomers and interaction of composites and saliva, may lead to formation of precipitates upon the surface of resin composites in the oral cavity. The type of storage solution had significant effect on the elution of monomers.
Methods: A UDMA- and BisGMA-containing nanohybrid composite resin was used (Evetric, Ivoclar Vivadent, Schaan, Liechtenstein). Four groups (n=10) were prepared for each storage solution: phosphate buffering solution, distilled water, artificial saliva and bicarbonate buffering solution. Teflon molds (diameter 10mm, thickness 2mm) were used for samples fabrication. Samples were cured using a LED light-curing unit (Bluephase, Ivoclar Vivadent, Schaan, Liechtenstein) and were stored in the tested solutions at 37o C in the dark. After 15 days of storage, 20 μl of the storage solution was removed and High Performance Liquid Chromatography (HPLC) was processed to analyze the elution of leached monomers. Data were analyzed with respect to the storage solution. The average amount of monomer elution in ppm and standard deviation were calculated for each storage solution. Statistical analysis was performed at level of significance p=0.05.
Results: No Bis-GMA and UDMA monomers were detected in the tested solutions after 15 days storage. However, Bisphenol A (BPA) was detected in phosphate and bicarbonate buffering solutions and distilled water.
Conclusion: Leaching of monomers and interaction of composites and saliva, may lead to formation of precipitates upon the surface of resin composites in the oral cavity. The type of storage solution had significant effect on the elution of monomers.