Bone regeneration, in the different tecnique. Immuno-histo-chemical exam

Method:

Our research protocol was approved on the basis of patients (in vivo) with partial or total atrophy who needed dental rehabilitation. In group I, 10 people were treated with blood Concentration Growth Factors; in group II, 10 people were treated with blood Concentration Growth Factors mixed with autologous bone; in group III, patients were treated with Hydroxyapatite, Tricalcium phosphate and bCGF (IAIO). After 6 months a follow-up biopsy was taken of the extracted tissue which was examined histologically and immune-histo-chemically using Anti-RUNX, Anti-Alkaline Phosphatase, Anti-SPARC and Ki67.

Result: The examination in II group showed that the best bone regeneration was detected among the patients treated with the biomaterial of blood Concentration Growth Factors and autologous bone. 

Conclusion:

Histological evaluation (HE) demonstrated the complete bone formation at the patients of group II, partial ossification for the patients of group I, and moderate ossification with persistent H/A residues of the patients of group III.

 During immuno-histo-chemical examination we obtained significant differences between group I, group II, and group III in terms of the formation of a new bone. 

To obtain a proper amount of bone regeneration for implant treatment, it is necessary to administer bCGF twice.