Methods: Subjects brushed with a commercial dentifrice (Aquafresh Advanced, 1150ppm F- as NaF, silica base) or a control dentifrice (250ppm F, same base), for 45, 60, 120 and 180 sec with 0.5 or 1.5 g dentifrice, and rinsed with 15 mL water either 1x or 3x, in a full cross-over design. Saliva samples were collected pre-brushing and over 120 min post-brushing, and their F- concentration determined (fluoride analysis of expectorate, rinse and toothbrush-washing samples will be reported elsewhere). The log area under the saliva fluoride clearance curve between 30-120 min post-brushing was used as a surrogate measure of retained F-.
Results:
Treatment |
Log10AUC30-120min |
Std. deviation |
1150ppm-45s-1.5g-1x15mL (n=38) |
0.94 |
0.301 |
1150ppm-60s-1.5g-1x15mL (n=38) |
0.95 |
0.324 |
1150ppm-120s-1.5g-1x15mL (n=40) |
1.00 |
0.289 |
1150ppm-180s-1.5g-1x15mL (n=40) |
1.00 |
0.296 |
1150ppm-45s-0.5g-1x15mL (n=41) |
0.69 |
0.363 |
1150ppm-60s-0.5g-1x15mL (n=39) |
0.69 |
0.316 |
1150ppm-120s-0.5g-1x15mL (n=40) |
0.80 |
0.418 |
1150ppm-60s-1.5g-3x15mL (n=41) |
0.86 |
0.295 |
250ppm-60s-1.5g-1x15mL (n=40) |
0.53 |
0.315 |
Dentifrice quantity and brushing duration (at the lower dose only) significantly affected retained F-. Over the ranges investigated, the numerical order of importance of the treatment variables was: F- concentration > dentifrice quantity > rinsing regimen > brushing duration (note no statistical comparisons between variables were performed).
Conclusions: Increasing brushing duration, and in particular dentifrice quantity, can elevate fluoride retention in the oral cavity post-brushing. Evidence is accumulating that the importance of these variables to fluoride efficacy may have been underestimated.