IADR Abstract Archives

Influence of Different Stressors on Periodontal Ligament Homeostasis

Objectives: Homeostasis of periodontal tissues is determined by cell differentiation, proliferation and apoptosis. Little is known about the effect of hypoxic, inflammatory and biomechanical stress on the turnover of the periodontal ligament (PDL). As previous studies have shown, the Insulin-like Growth Factor (IGF) system plays a key role in periodontal stress regulation. The objective of this study was to determine, if and how hypoxia, inflammation and biomechanical loading regulate proliferation, apoptosis and therefore homeostasis of human PDL cells.

Methods: PDL cells harvested from caries-free and periodontally healthy teeth were exposed to hypoxia (3% O2), inflammatory or biomechanical stress for 24 h. Interleukin-1 beta (IL-1β), which has been shown to be increased at inflamed periodontal sites, was used to simulate an inflammatory environment in vitro. In order to mimic functional loading of the periodontium, dynamic tensile strain (DTS, 3%, 1 Hz) was applied to cells. Unstimulated cells served as controls. Analysis of cell proliferation was performed by bromodeoxyuridine (BrdU) assay. Apoptosis was analyzed by a cell death detection ELISA. One-Way ANOVA and the post-hoc multiple comparison Tukey test were applied.

Results: All stressors had a remarkable influence on PDL homeostasis. Proliferation was altered significantly by hypoxia. In addition, apoptosis was regulated significantly by DTS and hypoxia. Hypoxia, DTS and the combined effect of IL-1β and DTS significantly changed gene expression of some members of the IGF system, e.g. IGF-1, IGFBP1  and IGFBP3.

Conclusions: The findings of our in-vitro study suggest that inflammatory, hypoxic and biomechanical stress can regulate proliferation and apoptosis of human PDL cells and thereby the homeostasis of PDL tissue. Our study suggests that the IGF system is part of that regulation in PDL cells.

Pan European Region Meeting
2012 Pan European Region Meeting (Helisinki, Finland)
Helisinki, Finland
2012
75
Scientific Groups
  • Memmert, Svenja  ( University of Bonn, Center of Dento-Maxillo-Facial Medicine, Faculty of Medicine, Bonn, N/A, Germany )
  • Götz, Werner  ( University of Bonn, Center of Dento-Maxillo-Facial Medicine, Faculty of Medicine, Bonn, N/A, Germany )
  • Deschner, James  ( University of Bonn, Bonn, N/A, Germany )
  • Gölz, Lina  ( University of Bonn, Center of Dento-Maxillo-Facial Medicine, Faculty of Medicine, Bonn, N/A, Germany )
  • Frede, Stilla  ( Clinic and Polyclinic for Anesthesia and operative intensiv care, Bonn, N/A, Germany )
  • Jäger, Andreas  ( University of Bonn, Center of Dento-Maxillo-Facial Medicine, Faculty of Medicine, Bonn, N/A, Germany )
  • Rath-deschner, Birgit  ( University of Bonn, Center of Dento-Maxillo-Facial Medicine, Faculty of Medicine, Bonn, N/A, Germany )
    • Oral Session
    Periodontal Research
    09/13/2012