Distribution of Streptococci in Oral Flora of Chimpanzee
Objectives: The oral flora in chimpanzee was investigated as a human evolutional model. The aim of this study was to investigate the oral flora with consideration of Streptococci.
Methods: Eleven chimpanzees (11 to 45 years-old) fed at the Primate Research Institute, Kyoto University were used. Oral plaque samples were collected using the sterilized tooth brush under anesthesia, during a routine medical check-up. The sample was inoculated onto Mitis-Salivarius (MS) agar and modified MS agar, and the grown colonies were analyzed by 16S rRNA gene sequences, and by microbiological and biochemical properties. The DNA was extracted from remain samples using the DNA extraction kit, and prepared for Roche 454-sequencing analysis by generating PCR products targeting the V5-V6 region of the 16S rRNA gene. The obtained sequences were analyzed by the Ribosomal Database Project Server.
Results: Forty three strains grown on MS and modified MS agar were obtained from 11 chimpanzees, and identified as S. troglodytae sp. nov (n=6), S. gordonii (n=6), S. dentirousetii (n=5) and others. Predominant phylum was Firmicutes (90-95%), and family Veillonellaceae was 38 to 97% determined by the pyrosequenceing method. On the other hand, family Streptococcceae was 0.06 to 11.5% within all phylotypes.
Conclusions: Family Veillonellaceae was considerably higher than family Streptococcceae. The chimpanzee could be an appropriated model considering the human oral health.
This study was supported by the Cooperation Research Program of Primate Research Institute, Kyoto University, and granted in part by JSPS (22592350).
Division: Pan European Region Meeting
Meeting:2012 Pan European Region Meeting (Helisinki, Finland) Location: Helisinki, Finland
Year: 2012 Final Presentation ID:704 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Imai, Susumu
( Tsurumi University, Yokohama, N/A, Japan
)
Miyabe-nishiwaki, Takako
( Kyoto University, Inuyama, N/A, Japan
)
Kaneko, Akihisa
( Kyoto University, Inuyama, N/A, Japan
)
Watanabe, Akino
( Kyoto University, Inuyama, N/A, Japan
)
Watanabe, Shohei
( Kyoto University, Inuyama, N/A, Japan
)
Hayashi, Misato
( Kyoto University, Inuyama, N/A, Japan
)
Tomonaga, Masaki
( Kyoto University, Inuyama, N/A, Japan
)
Hanada, Nobuhiro
( Tsurumi University, Yokohama, N/A, Japan
)
Okamoto, Masaaki
( Tsurumi University, Yokohama, N/A, Japan
)
Miyanohara, Mayu
( Tsurumi University, Yokohama, N/A, Japan
)
Saito, Wataru
( Tsurumi University, Yokohama, N/A, Japan
)
Momoi, Yasuko
( Tsurumi University, Yokohama, N/A, Japan
)
Abo, Tomoko
( Tsurumi University, Yokohama, N/A, Japan
)
Nomura, Yoshiaki
( Tsurumi University, Yokohama, N/A, Japan
)
Ikawa, Tomoko
( Tsurumi University, Yokohama, N/A, Japan
)
Ogawa, Takumi
( Tsurumi University, Yokohama, N/A, Japan
)
SESSION INFORMATION
Poster Session
Microbiology/Immunology II
09/15/2012