to grant in vivo changes in morphology and mineral composition of porous, calcium-phosphate deficient MIH molar after supplementation with a recently introduced calcium-phosphate fluoride product (CPP-ACFP, MI Paste Plus™, GC Europe, Leuven, Belgium). The study wants to establish that the same results, which were obtained previously after 3 years of application, can be achieved in 6 months time by adding fluoride to the CPP-ACP product previously tested.
Methods:
n=10 enamel fragments from newly erupted MIH molar cusps after PEB (post-eruption break-down) were taken in children from 6 to 7 years old (T0). Other n=10 samples were taken after 6 months (T3) of applications, smearing a pea size of product 5 times a day. The fragments were analysed by ESEM-EDX at low and high magnification (500X, 5000X). Following the EDX analysis, n=2 samples were gold-sputter: T0 before treatment, T3 after six months. Finally, both samples were SEM analysed at 2000X, 5000X, 20000X magnifications.
Results:
ESEM-EDX: after six months of applications, the concentration of Ca and P significantly (α< 0.05) increased and C decreased. As a result, the Ca/C levels can be compared to mineral proportion of normal enamel.
SEM: 20000X magnification images show that the affected crystals have acquired a more regular shape and that they are strictly organized. 5000X images present a reduction of porosity and interprismatic spaces, with well-packed mature enamel and evolution of defective rods of MIH molars into a more geometrical and mineralized shape of prisms.
Conclusions:
CCP-ACPF supplementation accelerates and increases the potential maturation of MIH enamel structure, which may or may not take place spontaneously over a much longer time, allowing post-eruptive break down prevention.