A Novel Mutans Streptococci isolated from Chimpanzee Oral Cavity
Objectives: We have been investigated the oral flora in chimpanzee as a human evolutional model. It was suggested that many novel species of bacteria harbored in oral cavity by the pyrosequencing method. In the present study, the taxonomic position of a novel mutans streptococci was investigated.
Methods: The isolates were identified based on the comparison of their partial 16S rRNA and two house keeping gene sequences. Phenotypic characterization was analyzed by API 50CH, API Rapid ID 32 Strep and API-ZYM systems.
Results: The colonies of isolates grown on Mitis-Salivarius agar were similar in morphology to that of Streptococcus mutans. Based on the partial 16S rRNA gene sequences of the isolates, the most closely related species was S. mutans (96.4%). The next closely related species to the isolates were S. devriesei (94.5%) and S. downei (93.9%). These isolates could be distinguished from S. mutans by the inulin fermentation and alkaline phosphatase activity (API-ZYM system). The isolates were not susceptible to bacitracin.
Conclusions: On the basis of the phenotypic characterization, partial 16S rRNA gene and two housekeeping gene (groEL and sodA) sequences data, we propose a novel taxa, Streptococcus troglodytae, (type strain =JCM 18038T =DSM 25324T) for this newly described isolate. These results might be developing the transmission and etiological studies of microbes associated with dental caries. This study was supported by the Cooperation Research Program of Primate Research Institute, Kyoto University, and granted by JSPS (22592350).
Division: Pan European Region Meeting
Meeting:2012 Pan European Region Meeting (Helisinki, Finland) Location: Helisinki, Finland
Year: 2012 Final Presentation ID:697 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Okamoto, Masaaki
( Tsurumi University, Yokohama, N/A, Japan
)
Miyabe-nishiwaki, Takako
( Kyoto University, Inuyama, N/A, Japan
)
Kaneko, Akihisa
( Kyoto University, Inuyama, N/A, Japan
)
Watanabe, Akino
( Kyoto University, Inuyama, N/A, Japan
)
Watanabe, Shohei
( Kyoto University, Inuyama, N/A, Japan
)
Hayashi, Misato
( Kyoto University, Inuyama, N/A, Japan
)
Tomonaga, Masaki
( Kyoto University, Inuyama, N/A, Japan
)
Hanada, Nobuhiro
( Tsurumi University, Yokohama, N/A, Japan
)
Imai, Susumu
( Tsurumi University, Yokohama, N/A, Japan
)
Miyanohara, Mayu
( Tsurumi University, Yokohama, N/A, Japan
)
Saito, Wataru
( Tsurumi University, Yokohama, N/A, Japan
)
Momoi, Yasuko
( Tsurumi University, Yokohama, N/A, Japan
)
Abo, Tomoko
( Tsurumi University, Yokohama, N/A, Japan
)
Nomura, Yoshiaki
( Tsurumi University, Yokohama, N/A, Japan
)
Ikawa, Tomoko
( Tsurumi University, Yokohama, N/A, Japan
)
Ogawa, Takumi
( Tsurumi University, Yokohama, N/A, Japan
)
SESSION INFORMATION
Poster Session
Microbiology/Immunology II
09/15/2012