Objectives: To determine if the addition of bioactive fillers to a dental adhesive may favour remineralisation at the resin-dentine interface.
Methods: Three experimental resin adhesives were applied onto phosphoric acid etched dentine: 1) neat resin, 2) resin with 90 wt% tri-calcium silicate mixed with 10 wt% β-Tri-Calcium phosphate (βTCS), 3) resin with polycarboxylated βTCP/ZnO-modified tri-calcium silicate (βZn-TCS). Molars were sectioned and resin-dentine bonded slices were stored in simulated body fluid (SBFS). After 24 hours and 3 months, five indentations were performed in wet condition along the interfaces (1-adhesive, 2-HL, 3-bottom HL, 4 and 5- dentine) using a AFM/Berkovich nanoindenter system. The modulus of elasticity (Ei) was evaluated. ANOVA/Student-Newman-Keuls analysis was performed (P<0.05). Confocal microscopy micropermeability (CLSM) was also performed.
Results: Mean (SD) of
Ei (GPa) are shown. Letters in columns and numbers in rows indicate significant differences. Mineral deposition reduced the micropermeability within the interfaces created using the resins containing βTCS or βZn-TCS bioactive filler after 3m of SBFS.
Conclusions: Adhesives containing the novel bioactive fillers facilitate HL remineralisation increasing Ei at the bonded interface.
Acknowledgements:CICYT/FEDERMAT2008-02347 and MAT2011-24551, JA-P08-CTS-3944 and P07-CTS-2568.
|
Resin 60 % )3TCS 40 %
|
Resin 60 % )3Zn-TCS 40 %
|
Neat resin
|
Ei - 24h
|
Ei – 3m
|
Ei - 24h
|
Ei – 3m
|
Ei - 24h
|
Ei – 3m
|
1st
|
4.02±1.1A1
|
3.85±1.1 A1
|
3.13±0.5A1
|
1.91±0.5 A2
|
2.81±0.5 A1
|
2.11±0.3 A1,2
|
2nd
|
1.42±0.5B1
|
2.95±0.7 B2
|
1.58±0.5B1
|
3.17±0.1 B2
|
1.22±0.2 B1
|
0.00±0.0B3
|
3rd
|
9.74±1.9C1
|
11.58±1.2C2
|
8.33±1.8C1
|
12.21±1.7C2
|
7.57±1.2 C1
|
7.54±1.4 C1
|
4th
|
17.9±1.8D1
|
17.9±1.0 D1
|
17.4±1.4D1
|
17.2±1.3 D1
|
17.8±2.1 D1
|
17.3±1.5 D1
|
5th
|
17.8±0.9D1
|
17.4±0.9 D1
|
17.9±1.2D1
|
17.9±1.7D1
|
17.9±1.2 D1
|
15.5± 1.0D1
|