Anti-inflammatory and Antibacterial Activities of Oxyresveratrol Isolated from Artocarpus lakoocha

Methods: The antibacterial activity of oxyresveratrol on P. gingivalis and A. actinomycetemcomitans was tested by using disc diffusion method. To study the anti-inflammatory activity of oxyresveratrol, LPS from P. gingivalis was used to mimic the inflammatory condition of HPDL cells. The mRNA expression and protein secretion of various cytokines were measured by RT-PCR and ELISA assay respectively.

Results: In disc diffusion assay, 4 mg/ml of oxyresveratrol was able to inhibit both P. gingivalis and A. actinomycetemcomitans. The clear zone size of oxyresveratrol in both bacteria was comparable to that of chlorhexidine, an antiseptic commonly used for dental plaque control. For inflammatory stimulation, LPS treatment strongly induced IL-6 and IL-8 expression in HPDL cells. However, IL1β and TNFα secretions were barely detectable, implied that these two cytokines were not mainly produced by HPDL cells. Treatment of oxyresveratrol significantly inhibited both IL-6 and IL-8 secretion. The inhibitory acitivity of oxyresveratrol also observed at mRNA level, suggested that oxyresveratrol might inhibit IL-6 and IL-8 production at the transcription process. In addition, oxyresveratrol did not cause any detrimental effect on HPDL cells

Conclusions: Our results indicated that oxyresveratrol has anti-bacterial activity against main oral pathogens related to periodontal disease. In addition, it also has anti-inflammatory activity on LPS-stimulated HPDL cells. Thus, oxyresveratrol might be a potential candidate to be used as anti-periodontitis agent.