IADR Abstract Archives
Collagen Sets the Foundation for Bone Vascularization and Regeneration.
Abstract Body:
In critical size defects, bone regeneration is facing the real challenge of inducing rapid vascular ingrowth and bone marrow stem cell recruitment.
Dual-phase bone substitutes are frequently used as therapeutic scaffolds for post-extraction ridge preservation and for bone regeneration in critical-size bone defects.
In order to understand how this type of materials is involved in bone regeneration, we investigated the angiogenic potential of xenogenic dual-phase bone substitutes containing native collagen of porcine and equine origins and their effects on bone marrow mesenchymal stem cell recruitment.
Our data showed that the application of these xenogenic substitutes significantly enhanced the release of Complement Bioactive C5a molecule from injured periodontal ligament cells (PDL) which induced bone stem cell proliferation and recruitment. These xenogenic materials also significantly induced angiogenic growth factors release from PDL cells, enhanced endothelial cell proliferation as well as angiogenesis as demonstrated by an increased formation of capillary-like structures. Our study also demonstrated that stem cell proliferation and recruitment was significantly higher with collagen-containing materials than without collagen.
Taken together, xenogenic bone substitutes which contain native collagen, provide structural and biological support for vascularization and bone regeneration by enhancing neo-angiogenesis and mesenchymal stem cell recruitment. These are major pre-requisites for bone regeneration in critical size defects.
In critical size defects, bone regeneration is facing the real challenge of inducing rapid vascular ingrowth and bone marrow stem cell recruitment.
Dual-phase bone substitutes are frequently used as therapeutic scaffolds for post-extraction ridge preservation and for bone regeneration in critical-size bone defects.
In order to understand how this type of materials is involved in bone regeneration, we investigated the angiogenic potential of xenogenic dual-phase bone substitutes containing native collagen of porcine and equine origins and their effects on bone marrow mesenchymal stem cell recruitment.
Our data showed that the application of these xenogenic substitutes significantly enhanced the release of Complement Bioactive C5a molecule from injured periodontal ligament cells (PDL) which induced bone stem cell proliferation and recruitment. These xenogenic materials also significantly induced angiogenic growth factors release from PDL cells, enhanced endothelial cell proliferation as well as angiogenesis as demonstrated by an increased formation of capillary-like structures. Our study also demonstrated that stem cell proliferation and recruitment was significantly higher with collagen-containing materials than without collagen.
Taken together, xenogenic bone substitutes which contain native collagen, provide structural and biological support for vascularization and bone regeneration by enhancing neo-angiogenesis and mesenchymal stem cell recruitment. These are major pre-requisites for bone regeneration in critical size defects.