IADR Abstract Archives
Endodontic Microbiome and its Association With Systemic Inflammatory Burden.
Objectives: Apical periodontitis in endodontic disease is inflammation affecting periapical area of tooth. It can contribute to a low-grade systemic inflammation which might impact patient’s systemic health. This study is aimed to characterize endodontic microbiome and identify it's associations with systemic biomarkers profile.
Methods: Participants were recruited [(n=35 Re-RCT Group), (n=50 healthy controls)]. Preoperative whole blood and serum samples were collected from control and Re-RCT Group and Intraoperative intracanal samples were collected from the Re-RCT group. Microbiome of blood and intracanal samples were investigated by targeted partial 16SrRNA gene-sequence analysis (V1-V2 hypervariable region) using illumine-MiSeq300 platform. Serum biomarkers profile of 15 analytes were investigated using Multiplex-microbead immunoassay. Microbiome analysis was carried out using DADA2 (version 1.2), R (version 4.1) and Human Oral Microbiome Database (HOMD) (version 15.1). Non-linear association was assessed using Spearman’s correlation and linear associations using Pearson’s correlation. Regression models were used.
Results: The prevalent genera isolated from Pre-operative blood samples included Cutibacterium, Staphylococcus, Corynebacterium, Bacillus, Streptococcus, Acinetobacter, Afipia, Sphingomonas, Novosphingobium, Prevotella, Capnocytophaga, Pseudomonas, Mycobacterium, Actinomyces, and Lactobacillus. The prevalent genera identified from the intracanal samples included Enterococcus, Streptococcus, Bacteroidaceae, Lactobacillus, Bacillus, Prevotella, Actinomyces, Tannerella, Flavitalea, Pseudomonas, Bacteoidetes, Pesudopropionibacterium, Fusobacterium, Afipia, Parvimonas, Porphyromonas, Dialister, Enterobacter, and Olsenella. The serum biomarkers profile comparison showed significant elevation in the serum levels of IL1b, CRP, FGF-23 and ADMA in Re-RCT group as compared to control group. Positive associations were present between blood Corynebacterium and ICAM1, and Novosphingobium and FGF23. Significant positive association were present between intracanal Bacteroidaceae (G-1) and ADMA, Parvimonas and ADMA and Enterobacter and FGF23.
Conclusions: The study results provide evidence of association of endodontic disease and inflammatory markers which may adversely impact patient’s systemic health.
Methods: Participants were recruited [(n=35 Re-RCT Group), (n=50 healthy controls)]. Preoperative whole blood and serum samples were collected from control and Re-RCT Group and Intraoperative intracanal samples were collected from the Re-RCT group. Microbiome of blood and intracanal samples were investigated by targeted partial 16SrRNA gene-sequence analysis (V1-V2 hypervariable region) using illumine-MiSeq300 platform. Serum biomarkers profile of 15 analytes were investigated using Multiplex-microbead immunoassay. Microbiome analysis was carried out using DADA2 (version 1.2), R (version 4.1) and Human Oral Microbiome Database (HOMD) (version 15.1). Non-linear association was assessed using Spearman’s correlation and linear associations using Pearson’s correlation. Regression models were used.
Results: The prevalent genera isolated from Pre-operative blood samples included Cutibacterium, Staphylococcus, Corynebacterium, Bacillus, Streptococcus, Acinetobacter, Afipia, Sphingomonas, Novosphingobium, Prevotella, Capnocytophaga, Pseudomonas, Mycobacterium, Actinomyces, and Lactobacillus. The prevalent genera identified from the intracanal samples included Enterococcus, Streptococcus, Bacteroidaceae, Lactobacillus, Bacillus, Prevotella, Actinomyces, Tannerella, Flavitalea, Pseudomonas, Bacteoidetes, Pesudopropionibacterium, Fusobacterium, Afipia, Parvimonas, Porphyromonas, Dialister, Enterobacter, and Olsenella. The serum biomarkers profile comparison showed significant elevation in the serum levels of IL1b, CRP, FGF-23 and ADMA in Re-RCT group as compared to control group. Positive associations were present between blood Corynebacterium and ICAM1, and Novosphingobium and FGF23. Significant positive association were present between intracanal Bacteroidaceae (G-1) and ADMA, Parvimonas and ADMA and Enterobacter and FGF23.
Conclusions: The study results provide evidence of association of endodontic disease and inflammatory markers which may adversely impact patient’s systemic health.