IADR Abstract Archives
Ion-Release and Antibacterial Properties of Copper-Doped Bioactive Glass Containing Composites
Objectives: This study evaluated the ion-release and antibacterial effect against Streptococcus Mutans of newly developed experimental resin composites containing copper-doped mesoporous bioactive glass nanospheres (Cu-MBGN).
Methods: Cu-MBGN was synthesized using microemulsion-assisted sol-gel method. Eight experimental composites were designed using Bis-GMA/TEGDMA base. Cu-MBGN composites had 1, 5, or 10wt.% of Cu-MBGN, silanized nanosilica, and inert silanized microfillers up to 65wt% or 70wt%. Composites with 45S5 bioactive glass or silica were mixed as reference materials. Discoid specimens (d=3mm, h=2mm, n=3/gr) were light-cured (2x20s, 890 mW/cm2). The specimens were immersed in HEPES buffered solution for 28 days, and the calcium and copper ion release was measured with ICP-MS. The adherence of S.mutans to test materials was determined in a free-floating and biofilm-forming test setup. SEM-EDX analysis was performed to determine the filler particle and elemental distribution at the surface. Nano-CT was used to characterize the percentage of composites' total, open and closed porosities. The data were analyzed using two-way ANOVA and Tukey’s post-hoc test (α=0.05).
Results: Expectedly, only Cu-MBGN materials showed the dose-dependent Cu-release, with the highest values at 10% Cu-MBGN and the lowest at 1% Cu-MBGN. The highest Cu-release was achieved at 1-day, followed by a significant drop on days 3, 7, and 14, and another rise at 28-days. Accordingly, 10% Cu-MBGN had the best antibacterial effect, demonstrated by the lowest adherence of free-floating bacteria and the lowest biofilm formation. 45S5-containing materials showed, in contrast, the highest S.mutans adherence. Ca-release was the highest in bioactive controls containing 15% of 45S5 BG, which correlated with the highest number of open porosities at the surface. Cu-MBGN composites demonstrated the same dose-dependent behavior for Ca as for Cu, with a dominant spike at 1-day. SEM-EDX showed a homogeneous filler distribution of all the materials.
Conclusions: Cu-MBGN composites show better antibacterial properties than 45S5 BG composites but a sustained Ca-release.
Methods: Cu-MBGN was synthesized using microemulsion-assisted sol-gel method. Eight experimental composites were designed using Bis-GMA/TEGDMA base. Cu-MBGN composites had 1, 5, or 10wt.% of Cu-MBGN, silanized nanosilica, and inert silanized microfillers up to 65wt% or 70wt%. Composites with 45S5 bioactive glass or silica were mixed as reference materials. Discoid specimens (d=3mm, h=2mm, n=3/gr) were light-cured (2x20s, 890 mW/cm2). The specimens were immersed in HEPES buffered solution for 28 days, and the calcium and copper ion release was measured with ICP-MS. The adherence of S.mutans to test materials was determined in a free-floating and biofilm-forming test setup. SEM-EDX analysis was performed to determine the filler particle and elemental distribution at the surface. Nano-CT was used to characterize the percentage of composites' total, open and closed porosities. The data were analyzed using two-way ANOVA and Tukey’s post-hoc test (α=0.05).
Results: Expectedly, only Cu-MBGN materials showed the dose-dependent Cu-release, with the highest values at 10% Cu-MBGN and the lowest at 1% Cu-MBGN. The highest Cu-release was achieved at 1-day, followed by a significant drop on days 3, 7, and 14, and another rise at 28-days. Accordingly, 10% Cu-MBGN had the best antibacterial effect, demonstrated by the lowest adherence of free-floating bacteria and the lowest biofilm formation. 45S5-containing materials showed, in contrast, the highest S.mutans adherence. Ca-release was the highest in bioactive controls containing 15% of 45S5 BG, which correlated with the highest number of open porosities at the surface. Cu-MBGN composites demonstrated the same dose-dependent behavior for Ca as for Cu, with a dominant spike at 1-day. SEM-EDX showed a homogeneous filler distribution of all the materials.
Conclusions: Cu-MBGN composites show better antibacterial properties than 45S5 BG composites but a sustained Ca-release.