IADR Abstract Archives
Periodontal Ligament Stem Cells Protected Alveolar Bone During Tooth Movement
Objectives: To investigate whether the transplantation of human periodontal ligament stem cells (PDLSCs) can alter the rate of orthodontic tooth movement (OTM) and/or alveolar bone morphometry in a rat model.
Methods: PDLSCs were isolated from extracted teeth, characterized as mesenchymal stem cells and labeled with gold nanoparticles for in vivo cell tracking. 12 twelve week-old male Wistar rats were divided into (1) transplantation and (2) control group, both of which were subjected to OTM by applying a constant orthodontic force of 25cN between one of the upper first molars and a mini-screw. In the transplantation group, 1x106 labeled PDLSCs were embedded into Matrigel and transplanted via periodontal ligament injection. In the control group, a sham injection with only Matrigel was performed. In vivo micro-CTs were taken before and 31 days after orthodontic force application. OTM and bone morphometry were compared between both groups by two-way repeated measures ANOVA.
Results: No significantly differences were observed in OTM rate between the transplantation and control groups. However, bone mineral density, bone volume fraction, trabecular thickness and trabecular number of the alveolar bone in the transplantation group were significantly higher than in the control group 31 days after force application (P＜0.05).
Conclusions: Although the transplantation of periodontal ligament stem cells did not alter OTM rate, it might reduce bone loss and thus protect the alveolar bone during OTM.
Methods: PDLSCs were isolated from extracted teeth, characterized as mesenchymal stem cells and labeled with gold nanoparticles for in vivo cell tracking. 12 twelve week-old male Wistar rats were divided into (1) transplantation and (2) control group, both of which were subjected to OTM by applying a constant orthodontic force of 25cN between one of the upper first molars and a mini-screw. In the transplantation group, 1x106 labeled PDLSCs were embedded into Matrigel and transplanted via periodontal ligament injection. In the control group, a sham injection with only Matrigel was performed. In vivo micro-CTs were taken before and 31 days after orthodontic force application. OTM and bone morphometry were compared between both groups by two-way repeated measures ANOVA.
Results: No significantly differences were observed in OTM rate between the transplantation and control groups. However, bone mineral density, bone volume fraction, trabecular thickness and trabecular number of the alveolar bone in the transplantation group were significantly higher than in the control group 31 days after force application (P＜0.05).
Conclusions: Although the transplantation of periodontal ligament stem cells did not alter OTM rate, it might reduce bone loss and thus protect the alveolar bone during OTM.