IADR Abstract Archives
Anti-Bacterial Composite Inhibits Growth of Salivary Bacteria: an Ex-Vivo Study
Objectives: Studies suggest that the incidence of secondary caries is higher in composite restorations than in amalgams. Saliva contains a broad spectrum of endogenous bacteria, some of which are cariogenic bacteria that interact to form the dental plaque. The aim of this study is to test the ability of an anti-bacterial composite to inhibit the growth of this wide variety of salivary bacteria thus affect the occurrence of secondary caries.
Methods: Test was caried out in a 96-well, flat-bottom microtiter plate. Bottom of 4 wells were coated with tested restorative materials: Anti-bacterial composite Infinix (Nobio), composite Filtek (3M) and amalgam Spherodon-M (Silmet). Wells were rinsed with phosphate-buffered saline. Unstimulated saliva was collected from 30 participants and tested separately. Whole saliva (10µl) from each participant was placed at the center of each tested materials. Plate was incubated (1h, 37°C) to ensure interaction between salivary bacteria and tested material. Growth medium was added, then plate was incubated again (1h, 37°C). Supernatant was transferred to a new plate. Plate was inserted into a microplate spectrophotometer (20h, 37°C). Kinetic reading was taken every 20min, optical density was measured as an indicator of bacterial growth. Measurements were analyzed and plotted.
Results: Bacterial growth rate measured in wells of bacteria that interacted with anti-bacterial composite was on average 4.87 times slower compared to control composite and 3.86 slower compared to amalgam (p<0.001). Moreover, growth onset of bacteria at the same wells, was delayed compared to control composite and amalgam (2.25 times and 1.59 times respectively; p<0.01).
Conclusions: Anti-bacterial composite showed statistically significant inhibition of salivary bacteria growth. Various levels of this growth inhibition were observed in samples collected from 30 participants thus indicating the anti-bacterial composite has the potential to shift caries balance towards health in all participants.
Methods: Test was caried out in a 96-well, flat-bottom microtiter plate. Bottom of 4 wells were coated with tested restorative materials: Anti-bacterial composite Infinix (Nobio), composite Filtek (3M) and amalgam Spherodon-M (Silmet). Wells were rinsed with phosphate-buffered saline. Unstimulated saliva was collected from 30 participants and tested separately. Whole saliva (10µl) from each participant was placed at the center of each tested materials. Plate was incubated (1h, 37°C) to ensure interaction between salivary bacteria and tested material. Growth medium was added, then plate was incubated again (1h, 37°C). Supernatant was transferred to a new plate. Plate was inserted into a microplate spectrophotometer (20h, 37°C). Kinetic reading was taken every 20min, optical density was measured as an indicator of bacterial growth. Measurements were analyzed and plotted.
Results: Bacterial growth rate measured in wells of bacteria that interacted with anti-bacterial composite was on average 4.87 times slower compared to control composite and 3.86 slower compared to amalgam (p<0.001). Moreover, growth onset of bacteria at the same wells, was delayed compared to control composite and amalgam (2.25 times and 1.59 times respectively; p<0.01).
Conclusions: Anti-bacterial composite showed statistically significant inhibition of salivary bacteria growth. Various levels of this growth inhibition were observed in samples collected from 30 participants thus indicating the anti-bacterial composite has the potential to shift caries balance towards health in all participants.