Objectives: To analyze the effect of doped polymeric nanoparticles (NPs) with zinc and dexamethasone in dentin remineralization. Methods: Polymeric NPs of 250 nm in diameter were synthetized and after doped with dexamethasone (Dex) or Zinc (Zn) by immersion in a saturated aqueous solution. Sound dentin discs (1 mm in thickness) were acquired from human molars with informed consent from donors (1906/CEIH/2020). Specimens were metallographically polished and etched with 35% phosphoric acid. An ethanol suspension of NPs, Dex-NPs or Dex+Zn-NPs was applied on dentin surfaces. A control group without NPs was also included. Nanomechanical properties (i.e. complex modulus) were assessed using a Hysitron Ti Premier nanoindenter, after 24 h and 21 d of immersion in phosphate buffered saline solution. ANOVA, Student Newman Keuls and Student t tests were performed (p<0.05). After nano-DMA specimens were fixed in a solution of 2.5% glutaraldehyde in 0.1 mol/L sodium cacodylate buffer for 24 h and observed by scanning electron microscopy (SEM) to ascertain changes in dentin morphology. Results: After 24 h storage, the dentin specimens that were not covered with NPs attained the highest complex modulus in GPa (control=69.35), and the rest of the groups follow the trend: NPs (20.29)=Dex+Zn-NPs (19.10)>Dex-NPs(8.86). After 21 d, complex modulus mean values were as follow: control (91.14)Conclusions: After 21d Dex+Zn-NPs and Dex-NPs produced dentin remineralization. An improved in nanohardness, complex modulus and tubules closure were observed in both groups. This work was funded by the Ministry of Economy and Competitiveness and European Regional Development Fund (PID2020-114694RB-I00 MINECO/AEI/FEDER/UE). M.T.-O. is fellow FPU of the Ministry of Universities [FPU20/00450].
Division: Meeting:2022 Pan European Region Oral Health Congress (Marseille, France) Location: Marseille, France
Year: 2022 Final Presentation ID:P270 Abstract Category|Abstract Category(s):Dental Materials 2: Polymer-based Materials
Authors
Toledano, Manuel
( University of Granada
, Granada
, Spain
)
Toledano-osorio, Manuel
( University of Granada
, Granada
, Spain
)
Medina-castillo, Antonio L
( University of Granada
, Granada
, Spain
)
Osorio, Maria T
( Independent Researcher
, Granada
, Spain
)
Osorio, Estrella
( University of Granada
, Granada
, Spain
)
Financial Interest Disclosure: This work was funded by the Ministry of Economy and Competitiveness and European Regional Development Fund (PID2020-114694RB-I00 MINECO/AEI/FEDER/UE). M.T.-O. is fellow FPU of the Ministry of Universities [FPU20/00450].
Support Funding Agency/Grant Number: This work was funded by the Ministry of Economy and Competitiveness and European Regional Development Fund (PID2020-114694RB-I00 MINECO/AEI/FEDER/UE). M.T.-O. is fellow FPU of the Ministry of Universities [FPU20/00450].