IADR Abstract Archives
The Remineralizing Effects of Fissure Sealants Containing Bioactive Glass
Objectives: The aim of this study was to evaluate and compare the effect of commercial fissure sealants modified with bioactive glass (BAG) particles of different sizes (nano, micro, hybrid) on the demineralization resistance of the enamel and its contribution to the remineralization.
Methods: Forty-five caries-free permanent molars were separated from their roots and embedded in epoxy resin. Then they were randomly divided into 5 groups and artificial initial caries lesions were formed on the occlusal surfaces of the teeth with a demineralization solution. After demineralization, the hardness and elasticity modulus values of the samples were measured by nanoindentation analysis. Commercial fissure sealant (Helioseal F Plus (HFP), Ivoclar,Vivadent) was applied to the control group. The experimental fissure sealants were applied to 3 groups: MICRO-BAG (15 wt% of micro-sized-BAG+ HFP), NANO-BAG (15 wt% of nano-sized-BAG+ HFP), and HYBRID-BAG (a combination of micro- (7.5 wt%) and nano-sized (7.5 wt%) BAG+ HFP). Fissure sealants were not applied to the negative control group. A second nanoindentation analysis was performed after a 7-day pH cycle. Then % surface hardness recovery values were calculated. Data were analyzed using one-way ANOVA, and t-test at an overall level of significance of 5%.
Results: In all groups, the average nanohardness values after the pH cycle were higher than nanohardness values after demineralization. However, the difference was statistically significant only in the NANO-BAG group (p=0,036). The highest average surface hardness recovery value was in the MICRO-BAG group, followed by; HYBRID-BAG, control, NANO-BAG, and negative control groups. But there was no statistically significant difference between the groups (p=0,820). There was no statistically significant difference between the elastic modulus values after demineralization and after the pH cycle in all groups (p>0,05).
Conclusions: Nano-sized BAG particles added to fissure sealants increase the resistance of the enamel tissue adjacent to the fissure sealant against demineralization and significantly contribute to its remineralization.
Methods: Forty-five caries-free permanent molars were separated from their roots and embedded in epoxy resin. Then they were randomly divided into 5 groups and artificial initial caries lesions were formed on the occlusal surfaces of the teeth with a demineralization solution. After demineralization, the hardness and elasticity modulus values of the samples were measured by nanoindentation analysis. Commercial fissure sealant (Helioseal F Plus (HFP), Ivoclar,Vivadent) was applied to the control group. The experimental fissure sealants were applied to 3 groups: MICRO-BAG (15 wt% of micro-sized-BAG+ HFP), NANO-BAG (15 wt% of nano-sized-BAG+ HFP), and HYBRID-BAG (a combination of micro- (7.5 wt%) and nano-sized (7.5 wt%) BAG+ HFP). Fissure sealants were not applied to the negative control group. A second nanoindentation analysis was performed after a 7-day pH cycle. Then % surface hardness recovery values were calculated. Data were analyzed using one-way ANOVA, and t-test at an overall level of significance of 5%.
Results: In all groups, the average nanohardness values after the pH cycle were higher than nanohardness values after demineralization. However, the difference was statistically significant only in the NANO-BAG group (p=0,036). The highest average surface hardness recovery value was in the MICRO-BAG group, followed by; HYBRID-BAG, control, NANO-BAG, and negative control groups. But there was no statistically significant difference between the groups (p=0,820). There was no statistically significant difference between the elastic modulus values after demineralization and after the pH cycle in all groups (p>0,05).
Conclusions: Nano-sized BAG particles added to fissure sealants increase the resistance of the enamel tissue adjacent to the fissure sealant against demineralization and significantly contribute to its remineralization.