IADR Abstract Archives
Biomimetic Calcium Phosphate Coating on Medical Grade Stainless Steel
Objectives: Stainless steel (SSL) minicrew implants have been applied in orthodontic clinics. They are of excellent physical properties but relatively poor biocompatibility. It is unknown whether the biocompatibility of medical grade smooth stainless steel can be improved by modifying SSL with a biomimetic calcium phosphate coating with or without additional drugs.
Methods: Titanium (Ti) discs and SSL discs were used in this study. The immersion period in a biomimetic modified Tyrode solution (BMT) was 24h at 37 degree for Ti discs to form an amorphous layer. For SSL discs, the immersion periods were 0h, 12h, 24h, 36h and 48h respectively. After this procedure, the discs were immersed in a supersaturated calcium phosphate solution (CPS) for 48h at 37 degree to form a crystalline layer. Physical, chemical and biological properties of the BioCaP coating were analyzed and compared. In addition, to assess protein loading profile and surface wettability variations of the crystalline layer, bovine serum albumin (BSA) was incorporated as a model protein.
Results: The amorphous and crystalline layers both increased the wettability of SSL. With the increase of immersing period in BMT, the average roughnes of coating surface, the size of crystals, the amount of BSA incorporated, cell seeding efficiency and cell spreading area of the crystalline layer were significantly increased.
Conclusions: These in vitro results show that the BioCaP coating can improve biocompatibility of the smooth medical grade SSL and serve as a carrier system for bioactive agents. In vivo study will be carried out by mimic the orthodontic treatments in the future.
Methods: Titanium (Ti) discs and SSL discs were used in this study. The immersion period in a biomimetic modified Tyrode solution (BMT) was 24h at 37 degree for Ti discs to form an amorphous layer. For SSL discs, the immersion periods were 0h, 12h, 24h, 36h and 48h respectively. After this procedure, the discs were immersed in a supersaturated calcium phosphate solution (CPS) for 48h at 37 degree to form a crystalline layer. Physical, chemical and biological properties of the BioCaP coating were analyzed and compared. In addition, to assess protein loading profile and surface wettability variations of the crystalline layer, bovine serum albumin (BSA) was incorporated as a model protein.
Results: The amorphous and crystalline layers both increased the wettability of SSL. With the increase of immersing period in BMT, the average roughnes of coating surface, the size of crystals, the amount of BSA incorporated, cell seeding efficiency and cell spreading area of the crystalline layer were significantly increased.
Conclusions: These in vitro results show that the BioCaP coating can improve biocompatibility of the smooth medical grade SSL and serve as a carrier system for bioactive agents. In vivo study will be carried out by mimic the orthodontic treatments in the future.