IADR Abstract Archives
Biocompatibility, Physical and Chemical Analysis of Calcium Silicate-Based Endodontic Sealers
Objectives: The present study aimed to evaluate, in vivo, the biocompatibility and in vitro, physical, and chemical properties of a newly proposed pre-mixed material in comparison with two other calcium silicate-based sealers and an epoxy resin-based material.
Methods: Bio-C Sealer Ion+, Bio-C Sealer, EndoSequence BC Sealer, and AH Plus (as control) were evaluated for subcutaneous inflammatory response after animal implantation. After 7 and 30 days, the rats were euthanised and the tubes were removed along with the surrounding tissues for the histologic analysis. Material surface and chemical characterisation were analysed using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS) and Raman spectroscopy. According to ISO 6876/2012 standard, flow, setting time (in both dry and moist environments), solubility, radiopacity, and pH were also analysed. The parametric results were statistically analysed by ANOVA using Levene test and Bonferroni correction and the non-parametric by Kruskal-Wallis followed by the Dunn test (p<.05).
Results: The inflammatory response observed for the calcium silicate-sealers was greater after 7 days subsided after 30 days. Peaks of calcium, silicon, and radiopacifier were observed for the calcium silicate-based sealers in the SEM/EDS and Raman analysis. All materials exhibited flow values above 17 mm, fulfilling the ISO standard. Bio-C Sealer obtained the shortest setting time in both environments; however, regarding the solubility, Bio-C Sealer Ion+ exhibited similar results of those obtained for EndoSequence BC Sealer and Bio-C Sealer (p>.05) not fulfilling the ISO standard. All materials exceeded 7 mm Al of radiopacity and exhibited a decreasing alkalinity until 21 days.
Conclusions: Bio-C Sealer Ion+ exhibited comparable properties to the other pre-mixed sealers, including their high solubility not fulfilling the ISO standards. Long-term endodontic sealers stability should be expected to ensure successful clinical outcomes, thus high solubility is a major concern considering pre-mixed ready-to-use sealers.
Methods: Bio-C Sealer Ion+, Bio-C Sealer, EndoSequence BC Sealer, and AH Plus (as control) were evaluated for subcutaneous inflammatory response after animal implantation. After 7 and 30 days, the rats were euthanised and the tubes were removed along with the surrounding tissues for the histologic analysis. Material surface and chemical characterisation were analysed using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS) and Raman spectroscopy. According to ISO 6876/2012 standard, flow, setting time (in both dry and moist environments), solubility, radiopacity, and pH were also analysed. The parametric results were statistically analysed by ANOVA using Levene test and Bonferroni correction and the non-parametric by Kruskal-Wallis followed by the Dunn test (p<.05).
Results: The inflammatory response observed for the calcium silicate-sealers was greater after 7 days subsided after 30 days. Peaks of calcium, silicon, and radiopacifier were observed for the calcium silicate-based sealers in the SEM/EDS and Raman analysis. All materials exhibited flow values above 17 mm, fulfilling the ISO standard. Bio-C Sealer obtained the shortest setting time in both environments; however, regarding the solubility, Bio-C Sealer Ion+ exhibited similar results of those obtained for EndoSequence BC Sealer and Bio-C Sealer (p>.05) not fulfilling the ISO standard. All materials exceeded 7 mm Al of radiopacity and exhibited a decreasing alkalinity until 21 days.
Conclusions: Bio-C Sealer Ion+ exhibited comparable properties to the other pre-mixed sealers, including their high solubility not fulfilling the ISO standards. Long-term endodontic sealers stability should be expected to ensure successful clinical outcomes, thus high solubility is a major concern considering pre-mixed ready-to-use sealers.