Methods: The study of the effect of the ibuprofen on the cellular proliferation was carried out by using as treatment doses 5, 25 and 75 μM during 24 and 48 hours and the flow cytometry. The effect on the differentiation was determined by quantifying the osteocalcine levels in the culture supernatant by immunoassay radio after the treatment with 25 μM ibuprofen doses during 24 hours. The experiences were carried out fourfold and the t Student analysis was followed for the statistical analysis.
Results: The treatment by using ibuprofen during 48 hours with 5 and 25 μM doses produced significant cellular proliferation increases p<0.05 and p<0.01, respectively, with no change in the treatment during 24 hours. The use of a higher doses approximately 75 μM just in treatments during 24 hours caused a significant proliferation decrease regarding the non-treated cellules p<0.05. We have also observed that the short term treatment (4 hours) of the MG63 line caused a significant osteocalcine synthesis decrease.
Conclusions: The ibuprofen in therapeutic doses in short term treatments could be the choosing NSAID in those clinical situations in wich a rapid bone tissue formation is required, like odonthology treatments.