Angiogenesis is the process of new vessel formation. Growth factors including basic fibroblast growth factor (FGF-2), platelet-derived growth factor (PDGF), transforming growth factor beta-1 (TGFâ-1) and vascular endothelial growth factor (VEGF-A) are known to regulate this process. Previous studies suggest that these growth factors may be involved in the response of human dental pulps to orthodontic force application. Objectives: The aim of this study was to identify the presence of angiogenic growth factors within pulps of control and orthodontically treated teeth. Methods: In patients, an orthodontic force (0.5 – 1 N) was applied to premolar teeth for 2 weeks. Contralateral teeth were used as untreated controls. Teeth were extracted, pulps were removed and RNA was extracted for the purpose of reverse transcription (RT-) and qPCR. MG-63 osteosarcoma cells were grown under standard conditions for use as a positive control. Primers were designed using Primer Express. Results: RT-PCR using MG-63 cells and control pulp samples indicated expression of angiogenic growth factors: FGF-2, PDGF, TGFâ-1 and VEGF-A. qPCR performed on control and test samples using FGF-2, PDGF, TGFâ-1 and VEGF-A primers indicated a varied individual response in angiogenic growth factor expression after 2 weeks of orthodontic force application. Previous studies showing an increase in the microvasculature of pulp explants from teeth treated with an orthodontic force for 2 weeks have suggested an increase in angiogenic growth factor expression. Shorter durations of orthodontic force application will be used in order identify the time point at which this suggested increase in angiogenic growth factor expression occurs. Conclusions: The present study identified the expression of angiogenic growth factors within the human dental pulp. qPCR results suggest a varied individual response in growth factor expression occurs after a duration of 2 weeks of orthodontic force application.
We acknowledge the King's Dental Institute Research Studentship Programme for funding.
Division: Pan European Federation Meeting
Meeting:2008 Pan European Federation Meeting (London, England) Location: London, England
Year: 2008 Final Presentation ID:238 Abstract Category|Abstract Category(s):PEF IADR 2008
Authors
Willson, Timarah Grace
( King's College London, London, N/A, United Kingdom
)
Silver, Nicholas
( King's College London, London, N/A, United Kingdom
)
Proctor, Gordon Burgess
( NIHR Biomedical Research Centre & Salivary Research Unit, London, N/A, United Kingdom
)
Derringer, Kathryn A.
( King's College London, London, N/A,
)
SESSION INFORMATION
Poster Session
Oral, Facial and Pulp Biology
09/10/2008