Objectives: Surface Modification of Titanium (Ti) implants, aiming at increasing the osteoconductivity of implant materials, has generated much attention in the research field of dental implantology. Our objective was to evaluate the cellular response of Ti modified surfaces between novel silicon-based anodic spark deposition (ASDSi) and electrohydrodynamic atomisation (EHDA) deposition. The biological activity was assessed using two human cell models; primary osteoblast (HOB) and mesenchymal stem cells (MSCs). Materials and Methods: Two different Ti surfaces were investigated: ASDSi and EHDA. Commercially pure Ti (cpTi) was the non-treated control surface. Test samples were cut into discs (12 mm in diameter and 0.5 mm thickness) and sterilized by γ-irradiation prior to the test. All surfaces were scanned using scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDS), and atomic force microscopy (AFM) for topographic and physicochemical characterization. HOB cells and MSCs were cultured in Dulbeco's Modified Eagle Medium with 10% foetal calf serum and seeded at a cell density of 1 x 105 cells/ml, directly onto the test discs (ASDSi, EHDA, and cpTi), as well as on Thermanox®, a negative control (non-toxic material), and polyvinylchloride (PVC), a positive control (toxic material). Test samples were incubated at 37 oC and 5% CO2. At day 1, cells were fixed and processed for SEM to assess morphology. Cell proliferation was assessed using the alamarBlue™ assay for 28-day period. Results: Both treatments altered surface topography, and surface chemistry of Ti implants. SEM images showed abundant cell layers on all surfaces with active cells visible, and cell filopodia extending across surface. All test samples showed comparable proliferation for both cell types compared to controls. Conclusion: This study has demonstrated that both novel modified Ti surfaces had nanotopographic texture, and chemically-treated composition, favouring cell attachment and growth. This property would be beneficial for enhancing osseointegration for dental implants.