IADR Abstract Archives

TGF-ß1 and TGF-ß3 in MEE Cell Death During Palate Fusion

During palate fusion, the midline epithelial seam (MES) cell death is a crucial event for its disappearance. At this time point, both TGF-ß1 and TGF-ß3 are expressed in this epithelium. Objectives: The aim of this work has been to determine the relationship between TGF-ß1 and TGF-ß3 with the cell death observed in the MES prior to palate fusion. Methods: We have performed double labelling of embryonic day 14.5 (E14.5) mouse palates with antibodies against TGF-ß1 and TGF-ß3 and TUNEL, E13.5 wild type or tgf-ß3 null palatal shelf cultures, added or blocked TGF-ß1 in these cultures, and in situ hybridization with tgf-ß1 and tgf-ß3 riboprobes. Results: Our results demonstrate that, in the wild type mouse, MES dead cells are TGF-ß1 positive and TGF-ß3 negative. However, both in the presence or absence of TGF-ß1, cell death only occurs if TGF-ß3 is present. The expression of tgf-ß3 is induced both by the exogenous addition of TGF-ß1 or its neutralization. Conclusion: We thus think that TGF-ß3 is the death inductor of MES cells, and that TGF-ß1 modulates its action. This work has been supported by grants from CM (GR/SAL/0539/2004), FIS (PI03/0185) and UCM/CM to the research group 920202.
Pan European Federation Meeting
2006 Pan European Federation Meeting (Dublin, Ireland)
Dublin, Ireland
2006
141
Scientific Groups
  • Murillo, Jorge  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Maldonado, Estela  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Martínez-Álvarez, Concepción  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Del Río, Aurora  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Barrio, Mª Carmen  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Garcillán, Beatriz  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Amorós, María  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Fuerte, Tamara  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Fernandez, Álvaro  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Martínez-sanz, Elena  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Paradas, Irene  ( Universidad Complutense Madrid, Madrid, N/A, Spain )
  • Poster Session
    G. Craniofacial Biology, Poster Session
    09/13/2006