Comparison of antibacterial activity of different self-etching primers and adhesives

Objectives:Several species of bacteria have been isolated from dental plaque associated with caries lesions there is evidence that Streptococcus and Lactobacilli are the major human dental pathogens. Adhesive systems with anti-bacterial effects may inhibit secondary caries formation and pulp inflamation by eliminating residuel bacteria. The aim of this study was to compare the antibacterial activity of different self-etching primers and adhesives. Methods: The antibacterial effects of Clearfil Protect Bond Primer and Bonding agent, AdheSE Primer and Bonding agent, Adper Prompt-L-Pop, Futurabond, Clearfil Tri S Bond and Cervitec (positive control,1%chlorhexidine varnish) were tested against standard strain of Streptococcus mutans, Lactobacillus casei and Lactobacillus acidophilus using the disk diffusion method. Standard filter paper disks (n =5) impregnated with 20 microL of each material were prepared. After incubation at 37 degrees C for 48 hours at %5-10 CO2 atmosphere, the diameter of inhibition zones were measured in millimeters. Data were analyzed using one way ANOVA and MANOVA. Duncan's Multiple Range Test was used for pairwise comparison. Results: The size of inhibition zones produced by adhesives varied among the brands. AdheSE Primer: S. mutans (20.6±1.51); L. casei (14.8±1.78); L. acidophilus (11.4±0.54). Adper Prompt-L-Pop: S. mutans (19.6±1.51); L. casei (13.8±1.64); L. acidophilus (13.8±1.09). Cervitec: S. mutans (23±0.00); L. casei (27±0.70); L. acidophilus (22.4±0.54). Clearfil Protect Bond Primer: S. mutans (17±0.00); L. casei (17.6±0.54); L. acidophilus (22.4±0.54). Futurabond was found effective only against S. mutans (14.6±1.67). Of all the materials tested; AdheSE Bonding agent, Clearfil Protect Bond Bonding agent and Clearfil Tri S Bond exhibit no inhibition zone(-)for all bacteria tested. Conclusion: Among the adhesives tested Clearafil Protect Bond Primer based upon MDPB was found to be most potent material against L. acidophilus. AdheSE primer and Adper Prompt-L-Pop are highly effective to S.mutans. For L.casei, Clearafil Protect Bond Primer is the most effective agent.