IADR Abstract Archives

Salivary Trefoil Factor 3 (TFF3) enhance migration of oral keratinocytes

Objectives: TFF3 is a small peptide (7kDa), secreted from gl. submandibularis. This peptide is one of three members of the Trefoil Factor Family (TFF). From studies of the gastro-intestinal tract it has been clearly shown that the TFFs play an important role in maintaining and protecting the mucosal surfaces. TFF3 promotes restituition, i.e. the rapid movement of neighbouring epithelial cells into a wound area. We wanted to elucidate the potential positive role of TFF3 in oral wound healing.

Methods: We performed a wound healing assay on keratinocytes isolated from oral mucosa. Cells in serum-free medium at passages 2-4, were grown to confluence. The monolayer was wounded with the tip of a 200?l-pipette, washed carefully, and allowed to migrate for 12hrs after stimulation with 0,25 and 0,5?M TFF3. The wounded areas were photographed before and after migration, and area recovered by migrating cells was measured. To demonstrate that the observed cell movement was not related to an increased number of cells, we added mitomycin C to the cultures (an inhibitor of mitosis) prior to wounding and stimulation. Conversely, to demonstrate that the cells actively migrated, we added cytochalasin B to the cultures (an inhibitor of migraton), prior to wounding and stimulation.

Results: Cells stimulated with TFF3 showed a higher migration index as compared with unstimulated controls(P=0,005, t-test). Treatment with mitomycin C did not impair the observed cell migration. This indicates that the movement is independent of mitosis. Treatment with cytochalasin B completely inhibited cell movement.

Conclusions: Our results show that TFF3-stimulation of normal keratinocytes promote migration. Based on our findings, we hypothesize that TFF3 is one of many factors involved in the rapid wound healing of the oral mucosa.


Pan European Federation Meeting
2006 Pan European Federation Meeting (Dublin, Ireland)
Dublin, Ireland
2006
720
Scientific Groups
  • Storesund, Trond  ( University of Oslo, Oslo, N/A, Norway )
  • Hayashi, Katsuhiko  ( University of Oslo, Oslo, N/A, Norway )
  • Khuu, Cuong  ( University of Oslo, Oslo, N/A, Norway )
  • Helgeland, Kristen  ( University of Oslo, Oslo, N/A, Norway )
  • Schenck, Karl  ( University of Oslo, Oslo, N/A, Norway )
  • Bryne, Magne  ( University of Oslo, Oslo, N/A, Norway )
    • Poster Session
    K. Oral Medicine & Pathology Poster Session II
    09/15/2006