Effect of various mouthrinses on the rheological characteristics of saliva.
Jurga-Krokowicz J.1, Paszynska E.1*, Deregowska-Nosowicz P.1, Czarnecka B.1, Leszczynski R.2
1Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, Poland
2Institute of Physics, Adam Mickiewicz University in Poznan, Poland
Objectives: To investigate the effectiveness of 5 mouthrinses (Table 1) on salivary viscosity, flow and pH.
Methods: 3 saliva samples were collected from each of 15 healthy volunteers. Sample t1 - unstimulated saliva expectorated over 15 min, t2 - stimulated saliva collected at 2 min and t3 - 15 min after rinsing the oral cavity with each of 5 mouthrinses (A-E).The procedure was repeated with distilled water (DW) as control. The viscosity of all the salivary samples was measured in Haake Caber1 (Thermo Elektron Corp., Germany) capillary extensional rheometer with laser micrometer (780nm wavelength) repeated 5 times making 450 measurements. Salivary flow was measured gravimetrically (Radwag, WPA 60, Poland) and the pH by a single pore electrode (Hamilton AG, Switzerland).
Results: There was a significant difference between the viscosity of unstimulated and stimulated saliva (Wilcoxon matched pair test, p<0.03).There were significant differences in salivary viscosity after rinsing with 0.2% and 0.1% CHX mouthrinses (A,B,C), with B(0.1%) being effective longer. We found only a linear relationship among the 3 variables: increase of viscosity, flow and pH in mouthrinse D.
|
Mouthrinses |
Salivary viscosity (mPaxs)(t1) (mean±SD) |
Salivary viscosity(mPaxs)(t2) (mean±SD) |
Salivary viscosity (mPaxs)(t3) (mean±SD) |
|
DW (distilled water) |
0.8 ± 0.2 |
0.85 ± 0.3 |
1.37 ± 1.5 |
|
A (Corsodyl-0.2% CHX) |
1.31 ± 1.4 |
2.25 ± 1.8 |
1.17 ± 0.9 |
|
B (Kin Gingival-0.1% CHX) |
1.78 ± 1.1 |
2.16 ± 1.6 |
3.11 ± 2.2 |
|
C (Eludril 0.2% CHX) |
1.74 ± 1.1 |
1.58 ± 1.2 |
0.87 ± 0.5 |
|
D (Listerine-essential oils) |
1.91 ± 1.3 |
1.76 ± 1.8 |
2.1 ± 2.0 |
|
E (Oral B-0.05% NaF) |
1.23 ± 0.8 |
1.76 ± 1.4 |
2.23 ± 1.9 |
Conclusion: The mouthrinses, depending on their content, have a different impact on the viscosity of saliva. The use of this extensional rheometer can provide unambiguous information on the capillary breakup of viscoelastic saliva threads.