Components of Dentin Bondings Effect Host Cells and Microorganisms

Objectives: Dental materials are incorporated in oral cavity and interact both with host tissues and a high number of different microorganisms. Growth and adhesion of pathogenic bacteria should be avoided.The purpose of our study was to gain knowledge about the effects of components of dentin bondings on host cells and bacteria. Methods: Eluates from Syntac Classic® (adhesive and primer), AdheSE (adhesive and primer), Xeno III®, Heliobond® as well as from HEMA and TEGDMA had been made in PBS. Suspensions of Streptococcus mutans ATCC 25175, S. sanguinis ATCC 10556, Enterococcus faecalis ATCC 29212, Actinomyces viscosus 15987, and Porphyromonas gingivalis ATCC 33277 were added to different dilutions of the eluates and incubated for 6 h. Then the numbers of viable bacteria were enumerated. Reactions of host cells (fibroblasts of gingiva and pulp) were tested by means of MTT detection. Furthermore bondings of Syntac Classic and Xeno III were put into cavities of teeth. Adhesion of S. mutans and of a mixture of S. sanguinis, Fusobacterium nucleatum ATCC 25586, A. viscosus, and P. gingivalis was determined. Results: Especially the primers of the bondings in a dilution up to 1 : 40 containing several components suppressed the growth of oral microorganisms. Heliobond had no antibacterial activity. TEGDMA reduced only the number of viable P. gingivalis. In general cytotoxic reactions of the gingival and pulp fibroblasts were found until a dilution of 1 : 80. The lowest MTT-values were measured after addition of AdheSE (primer), HEMA and TEGDMA. Determination of cfu as well as SEM photographs showed a higher number of bacteria on Xeno III as on the Syntac Classic belonging to the conventional group of bondings. Conclusion: Bondings are able to effect host cells and they influence the growth of oral microorganisms. The surface of the classical bondings might be favourable in preventing adhesion of bacteria.