Methods: The nanostructured surface of medical Ti6Al4V disks was prepared by supersonic fine particles bombarding (SFPB) technique. The nanostructured surface was characterized by scanning electron microscopy and transmission electron microscopy. The control group of medical Ti6Al4V disks was treated with sulfuric acid and hydrochloric acid. Osteoblasts (MG63) were cultured and seeded on the disks. Cell adhesion was observed with florescence microscopy (Acridine Orange staining) at 8h, 24h later.
Results: SEM showed the concave pits with sub-micro diameters, rough and complicated microtextures on the titanium surface using SFPB. The nanostructured surface layer up to tens of micrometers thick was produced on Ti6A14V. The adhered osteoblasts on the nanostructured surfaces were obviously more than those on the acid etched surfaces, and the spreading of adhesion cells on the nanostructured surfaces were better than those of control group.
Conclusions: The nanostructured titanium surface is more beneficial to the osteoblasts adhesion than the acid etched surface.