Osteoconductivity of Injectable: Bone Xenograft, Hydroxyapatite, and Hydroxyapatite-Chitosan

Objective: The purpose of this study was to assess osteoconductivity of an injectable bone xenograft (IBX), injectable hydroxyapatite (IHA) and injectable hydroxyapatite-chitosan (IHA-C) on osteoblastic cell line (MG-63). Bone graft in the form of injectable paste gives several advantages over the powder form as it could be placed in the defect area that has limited accessibility. Methods: Three concentrations (0.25%, 0.5% and 1.0%) of IBX, IHA and IHA-C were supplemented with DMEM culture medium. The osteocalcin production by cells culture were measured by ELISA technique. The concentration of osteocalcin was measured at day 1, 3, 5, 7 and 14. Results: The highest level of Osteocalcin secretion of all test groups was found on the 7 day of exposure. The highest level of osteocalcin in the IBX paste group was 0,25% concentration while the highest level of osteocalcin in the IHA-C and IHA paste group was 1% and 0,25%, respectively. Conclusion: It was suggested that adequate osteoconductivity was evident for all injectable pastes tested.