Antitumoral Immunity by OK-432-conjugated Tumor Vaccine in Mice Cancer Model
Objective: The OK-432, which is a penicillin-inactivated streptococcal preparation, was used as a foreign antigen for conjugated with tumor cells for augmentation of tumor antigenicity. We have previously documented that OK-432-conjugated tumor vaccine (KLN-205 vaccine) strongly suppressed tumor incidence and growth, and improved survival rate as tumor-specific immunity in murine tongue cancer model. The purpose of the research is to clarify the mechanisms of antitumor immune response elicited by KLN-205 vaccine.
Methods: Six-week-old female DBA/2 mice were individually randomized, divided into three groups. KLN-205 tumor cells derived from the squamous cell carcinoma of DBA/2 mice. KLN-205 cells were mixed with OK-432 serviced as KLN-205 vaccine. The prepared KLN-205 vaccine was injected into mice once a week for 3 weeks, and splenetic cells from the mice were isolated and cultured with tumor cells. Cytokines in serum and culture supernatant were then measured using ELISA. Immunohistochemical analysis for CD4, CD8, CD25(IL-2Rá), and CD69 was performed by indirect immunoperoxidase staining.
Results: When the mice were immunized with the KLN-205 vaccine three times, higher IL-12, IL-18 and INF-ã levels were seen in serum and culture supernatant of splenetic cells than the control mice. After tumor inoculation in the KLN-205 vaccine group, a greater number of CD4 and CD8 T cells had infiltrated around tumor cells than the control group. Moreover immunohistochemical results showed that the expression of CD25(IL-2Rá) and CD69 in T cells were transiently higher by 24 hours in the KLN-205 vaccine group.
Conclusion: These results indicated that the KLN-205 vaccine induced tumor-specific cell-mediated immunity and suppress tumor activity.
Division: Asia/Pacific Region Meeting
Meeting:2009 Asia/Pacific Region Meeting (Wuhan, China) Location: Wuhan, China
Year: 2009 Final Presentation ID:591 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Li, Xianqi
( Institute for Oral Science, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Furusawa, Kiyofumi
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Uematsu, Takashi
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Yang, Jing
( Institute for Oral Science, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Takahashi, Miho
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Uchihashi, Takayuki
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Marukawa, Kazuya
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Akita, Daisuke
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Nakazawa, Takashi
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)
Teramoto, Yuji
( Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Nagano, N/A, Japan
)