Materials and methods:We used the chromophore DAF-FM DA for NO detection. Using combined atomic force microscopy and fluorescence microscopy, we report both stimulation and real-time monitoring of NO responses in single hPDLC induced by application of quantified periodic indenting forces to the cell membrane.
Results:
When mechanical stress was loaded in hPDLC, NO gradually reached peak within 10-15min and showed a certain mechanical stress strength-dependence. There was a significant difference in NO between 0.5 and 2.5 nN. NO increase can be inhibited by eNOS suppressor to some extent.
Conclusion:
Intracellular NO may be involved in hPDLC mechanical signal transduction caused by different mechanical stress.