Methods: Establish experimental tooth movement model with the Wistar rat,use normal rat as control.10 rats each group. After the force act 3 days, we got the blood mononuclear cells were isolated by density gradient centrifugation. Then the suspending cells were cultured in the selective culture medium for 2w. Immuocytochemistry and immunofluorescence and fluoresencent chemical technique were used to identificate these cells. EPCs labeled by BrdU were transplanted back into rat with tooth movement model by tail vein .
Results: Most of the mononuclear cells in experiment group stretched after cultured for 3 days.These cells proliferated faster and exhibited the line and clonal morphology after 7`10 days culture. The attached cells in experiment group took up DiI-ac-LDL ,bound FITC-UEA-1(double positive fluorescence) and positive express CD133, vWF and CD34. In the control group , the number of attached cells was fewer and they proliferated slower. Meanwhile, the cells positive express the character of endothelial cell is fewer too.We also found the EPCs can move to periodontal tissues during experimental tooth movement.
Conclusions: The quantity of EPCs will increase during experimental tooth movement. We have isolationed and cultured EPCs in peripheral blood in vitro and they can express the character of endothelial cell after induced.The EPCs can involved in the tissue remodel during tooth movement