Studies on Improving Osteoconductivity of Titanium by Hydrothermal Treatment
Purpose: As an implant material, titanium has been used widely in dentistry and orthopedics for 40 years. However, the bone bonding ability of Ti is much lower than those of osteoconductive materials such as hydroxyapatite, represented by delayed bone formation and fibrous tissue interposition at Ti-bone interface. Therefore the purpose of this study is to develop a hydrothermal treatment method and investigate its effect on improving the osteoconductivity of Ti implant materials.
Materials and methods: Pure Ti samples were hydrothermally treated in CaCl2 solutions for 24 h. Surface characteristics of the samples were analyzed by SEM and x-ray photoelectron spectroscopy. To reveal the bone cell responses to hydrothermally treated Ti, rat bone marrow cell experiments were performed. Cell attachment and cell proliferation ratio, osteoblast differentiation makers including alkaline phosphatase, osteocalcin, and extracellular matrix mineralization were investigated. A rodent tibia model was applied to determine the in vivo bone response to hydrothermally treated Ti surface. Histological observation was performed and bone contact ratio was measured.
Results: After hydrothermal treatment, the Ti sample presented a Ca-bonded oxide layer on the surface (Ca-Ti). Cell culture showed that Ca-Ti promoted cell adhesion and proliferation; accelerated the production of osteocalcin and the mineralization of the extracellular matrix. In animal experiment, Ca-Ti implants showed osteoblasts adhesion and new bone formation within 1 week. Bone contact ratio of Ca-Ti was much higher than untreated or alkali treated Ti from the early stage.
Conclusion: Hydrothermal treatment is a promising method for modifying Ti implant surfaces for accelerated osteointegration and skeletal fixation.
Division: Asia/Pacific Region Meeting
Meeting:2009 Asia/Pacific Region Meeting (Wuhan, China) Location: Wuhan, China
Year: 2009 Final Presentation ID:39 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Zhang, Lei
( School of stomatology, Tongji University, Shanghai, N/A, China
)
Nakagawa, Masaharu
( Kyushu University, Fukuoka City, N/A, Japan
)
Cong, Lei
( School of stomatology, Tongji University, Shanghai, N/A, China
)
Chen, Janrong
( School of stomatology, Tongji University, Shanghai, N/A, China
)
Ayukawa, Yasunori
( Kyushu University, Fukuoka, N/A, Japan
)
Legeros, Racquel Z.
( New York University, New York, NY, USA
)
Matsuya, Shigeki
( Fukuoka Dental College, Fukuoka, N/A, Japan
)
Koyano, Kiyoshi
( Kyushu University, Fukuoka, N/A, Japan
)
Ishikawa, Kunio
( Kyushu University, Fukuoka, N/A, Japan
)
SESSION INFORMATION
Oral Session
Oral Session D (Implantology Research & Dental Materials: Clinical Trials, Biocampatibility and Biologic Effects)
09/23/2009