Methods: Multiple blocks of paraffin-embedded tissue from resection specimens of patients with oral squamous cell carcinoma will be selected, who have had no previous chemotherapy or radiotherapy. Multiple samples from each specimen will be collected to minimize the limitation imposed by heterogeneity. Enzyme digestion to extract the nucleus. The comparison of the Nuclear Optical Density values of these cases that were studied successfully by Image Cytometry with Feulgen-stained tissue sections. The samples will be processed according to the image path system manufacturer specifications.
Results: preliminary results were found diploid and nondiploid (aneoploid and tetraploid), revealed a statistically significant difference between the control groups and both the tumour and margin groups (p < 0.01, not shown),
Conclusion: preliminary results suggest that the tumour tissue is considered to be diploid if the mean percentage of the tumour cells (nondiploid) does not exceed 10%. Otherwise it will be considered as aneoploid tumour tissue if the mean percentage of the tumour cells (nondiploid) is more than 10%.