Methods: The study was single blinded, randomized 4cell,cross over design. it used a 4 day plaque accumulation model to compare four different oral hygiene regimens with a wash out period of 7 days. 40 healthy volunteers were enrolled .The regimens were: using CHX mouthrinse before (Regimen A), immediately after (Regimen B), 30 minutes after tooth brushing (Regimen C) and only brushing (Regimen D) with SLS-containing dentifrice. at the end of each 4 day test period, plaque was scored with Turesky index. No other oral hygiene measures were allowed. The difference between the groups were analyzed by repeated measure of ANOVA test
Results: The overall indices of regimens A,B,C,D was 0.90,0.87,0.83,0.96 respectively. there was no significant difference in plaque accumulation between four regimens.
Conclusions: within the limitations of The present study it can be concluded that the anti-plaque efficacy of 0.2% CHX rinse was not reduced in combination of tooth brushing with SLS-containing dentifrice.