Methods: The randomly amplified polymorphic DNA profiles were applied to fingerprint 280 oral Candida strains isolated frown cancer patients receiving chemotherapy. For this purpose two different primers,RSD6 and RSD12 were used to develop a combination randomly amplified polymorphic DNA profile for each isolate.
Results: The two primers, RSD6 and RSD12,were successful in eliciting 17(A1-A17) and 2 (B1-B2) genotypes, respectively from among the 280 isolates . The genotypes generated from RSD6(A1-A17) and RSD12 (B1-B2)were then combined to generate the final genotype for each isolate. Altogether ,twenty-one genotypes were noted among the 280 isolates.
Conclusion: Saccharomyces albicans , especially genotypic subgroup A10B2 is the prevalence subpopulation in the oral Candida obtained from cancer patients receiving chemotherapy.And the genes of Candida albicans of clinic strains were stable in pathopoiesis procedure.