IADR Abstract Archives

Effect of storage media on material properties in extracted human teeth – A systematic review

Objectives: To study the effects of storage media on the results of materials being tested in an invitro studies on extracted teeth.
Methods: An electronic search was in four databases PubMed , CINAHL Plus, Dent & Oral Science and Cochrane databases along with manual searching. The PICO was kept as following; Population- Extracted Human Teeth, Intervention: Different Storage media, Comparison: Comparison of storage media amongst themselves , Outcome(s): Material property testing , Setting : In-vitro experimental studies.
Results: A total of 131 articles were found out of which 39 duplicates were removed. 95 articles were then screened from which 19 articles were shortlisted based on title .A total of 13 articles were then shortlisted based on title from which 12 articles were included in the systematic review process. Quinn tool was utilised to assess the risk of bias of invidual invitro studies , which revealed 2 studies out of 12 to be of low risk whereas remaining were of unclear risk
Conclusions: So far relatively fewer high quality studies are done which focus on the effect that storage media has on dental material properties. This review indicated that no material has yet found to be superior in terms of maintaining or improving the properties of tested materials.However, dry storage , formalin and cryopreservation were found out to be least affecting the properties of dental materials. Properly designed and controlled studies of higher quality are recommended to be conducted.

2023 Pakistan Section Meeting (Hybrid/Lahore, Pakistan)
Hybrid/Lahore, Pakistan
2023

Dental Materials 8: Clinical Trials
  • Billoo, Sadiq  ( AKUH , Karachi , Pakistan )
  • Raza Kazmi, Syed Murtaza  ( AKUH , Karachi , Pakistan )
    • NONE
    Oral Session
    Oral Presentations
    Overview of studies included
    1Bouroziniat et al 2017Storage duration had a negative effect
    		0.818 - storage media
    0.221 - autoclaving
    2De Roo et al 2020Low values of no significance
    		0.443
    3Silva et al 2015
    Bond strength values decreased with increased storage duration
    Mineral Oil and distilled water showed favourable bond strengths as compared to NaOCl
    0.75
    4
    		Mobarak et al 2010Use of human extracted teeth that are stored for up to 2 years, refrigerated in chloramine-T or stored dry and rehydrated prior to testing, does not affect the micro-tensile bond strength to dentin
    		>.05
    5De Souza et al 2008Long term storage significantly increased the presence of marginal gaps, which appeared in non-beveled restorations only<0.05

    6Branstorm et al 1992Teeth stored in chloramine solution resulted in more microleakage than those in freezed medium 0.0004
    7Tosun et al 2007Thymol showed lowest SBS values as compared to distilled water and formalin, distilled water showed highest SBS values>0.05
    8Toledano et al 2007Water showed a decrease in MTBS as compared to Dry and mineral oil storage < 0.0001
    9Camps et al 1996Prolonged cryopreservation did not affect microleakage whereas prolonged freezing did
    		0.001
    10Retief et al 1989
    Duration had no effect but ethanol and thymol lowered bond strength
    		Physiologic saline 0.1940
    1% chloramine 0.8640
    Formalin 0.3868
    0.05% thymol 0.3921
    70% ethanol 0.1565
    11Panighi et al 1997Except for superficial occlusal dentine, SBS values were similar between Fresh and cryopreserved teeth(0.0567)
    12Haller 1993Microleakage of chloramine and freshly extracted teeth was similar, formalin showed increased microleakage
    		0.0180

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