IADR Abstract Archives
Dentine Stain-minimization, Remineralization Potential and Morphological Aspects of Capping Agent-modified SDF-treated Dentine
Objectives: To investigate the effect of tannic acid (TA) and glutathione (G) incorporation in silver diamine fluoride (SDF) on stain-minimization, remineralization, antibacterial potential, and micromorphological properties of demineralized dentine.
Methods: TA and G were incorporated in 38% SDF (5,10,15%w/v) and applied to demineralized (0.5M-EDTA/30 minutes) bovine dentine (6×6×1mm3). Color (ΔE) (n=15), morphological and compositional changes (n=3) were recorded up to 90-day using color spectrometry, FESEM, and EDX. Antibacterial efficacy (n=8) against S. mutans was evaluated using agar disk diffusion test. Remineralization potential (RP) (n=5) was evaluated up to 6 months using FTIR. Data were statistically analyzed using parametric tests (p<0.05).
Results: At 90-day, TA- and G-treated specimens showed significant concentration-dependent reduction in ΔE compared to SDF, with TA being more effective.TA15 had the lowest ΔE (29.29±2.4) while SDF (53.4±2.6) and G10 (39.51±2.78) showed higher ΔE. The stain-minimization ability of capping agent modified-SDF at 90-day was in the following order: TA15>TA10>TA5>G15>G5>G10. At 6-month, TA15 had the highest RP (0.34±0.027) while G5 had the lowest (3.49±0.67). The highest zone of inhibition (ZOI) was exhibited by SDF (16.03±1.1mm), while deionized water (DI) and TA15 had the least (6.09±0.04mm, 7.29±0.16mm, respectively). The ZOI of pairs SDF-DI/SDF-TA10/SDF-TA15 was significantly different while the pairs SDF-TA5/SDF-G5/SDF-G10 and SDF-G15 had statistically non-significant mean ZOI. FESEM showed large silver (Ag) deposits (1−2 μm) on SDF-treated specimens which enlarged till 90-day. In contrast, nanosized Ag particles formed on TA5 and G5 and resisted size-change 90-day post-treatment. EDX analysis showed Ag as a major constituent in SDF and G5 at all timepoints while TA-modified specimens mainly contained calcium and phosphate.
Conclusions: TA- and G-incorporation in SDF significantly reduces tooth staining through Ag+ capping, particle size and chemical stabilization. Higher TA (5,10%) and G (10%) concentrations improve RP, which occurs at the cost of antibacterial efficacy, especially in TA-modified SDF.
Methods: TA and G were incorporated in 38% SDF (5,10,15%w/v) and applied to demineralized (0.5M-EDTA/30 minutes) bovine dentine (6×6×1mm3). Color (ΔE) (n=15), morphological and compositional changes (n=3) were recorded up to 90-day using color spectrometry, FESEM, and EDX. Antibacterial efficacy (n=8) against S. mutans was evaluated using agar disk diffusion test. Remineralization potential (RP) (n=5) was evaluated up to 6 months using FTIR. Data were statistically analyzed using parametric tests (p<0.05).
Results: At 90-day, TA- and G-treated specimens showed significant concentration-dependent reduction in ΔE compared to SDF, with TA being more effective.TA15 had the lowest ΔE (29.29±2.4) while SDF (53.4±2.6) and G10 (39.51±2.78) showed higher ΔE. The stain-minimization ability of capping agent modified-SDF at 90-day was in the following order: TA15>TA10>TA5>G15>G5>G10. At 6-month, TA15 had the highest RP (0.34±0.027) while G5 had the lowest (3.49±0.67). The highest zone of inhibition (ZOI) was exhibited by SDF (16.03±1.1mm), while deionized water (DI) and TA15 had the least (6.09±0.04mm, 7.29±0.16mm, respectively). The ZOI of pairs SDF-DI/SDF-TA10/SDF-TA15 was significantly different while the pairs SDF-TA5/SDF-G5/SDF-G10 and SDF-G15 had statistically non-significant mean ZOI. FESEM showed large silver (Ag) deposits (1−2 μm) on SDF-treated specimens which enlarged till 90-day. In contrast, nanosized Ag particles formed on TA5 and G5 and resisted size-change 90-day post-treatment. EDX analysis showed Ag as a major constituent in SDF and G5 at all timepoints while TA-modified specimens mainly contained calcium and phosphate.
Conclusions: TA- and G-incorporation in SDF significantly reduces tooth staining through Ag+ capping, particle size and chemical stabilization. Higher TA (5,10%) and G (10%) concentrations improve RP, which occurs at the cost of antibacterial efficacy, especially in TA-modified SDF.