IADR Abstract Archives
Effectiveness of EDTA versus SmearOFF on smear layer removal from root canal system: An In-vitro experimental study
Objectives: To compare mean smear layer removal from root canal space by measuring the area of methylene blue dye penetration in mm2 with TG-Cleanser (17% EDTA) versus SmearOFF TM (18%EDTA plus 1%Chlorhexidine) as a final irrigant under stereomicroscope.
Methods: It was an in-vitro experimental study. Sixty teeth were randomly allocated into two experimental groups. Teeth in each group were standardized to 16mm form root apex and crowns were removed. After chemo-mechanical preparation of roots with ProTaper Next rotary file system, till up to X3 file to standardize root canal flare, roots were divided into two experimental groups. After irrigation with final designated irrigant, 1% methylene blue dye was filled and dried with paper points from root canal. Each root was sectioned horizontally at 2, 5 and 8mm, representing apical, middle and coronal sections, respectively and examined under stereomicroscope (magnification X25.6). Area of dye penetration was measured using ImageJ software. Area of dye penetration in mm2 was recorded as the irrigant efficacy. Factorial design ANOVA was applied to compare area of methylene blue dye penetration between two groups (SmearOFF TM versus TG-Cleanser) at coronal, middle and apical root sections. A p-value of < 0.05 was taken as statistically significant.
Results: Teeth in TG-Cleanser showed a mean dye penetration of 1.69 ±1.3 mm2, whereas, SmearOFF showed a mean dye penetration 0.63 ±0.7 mm2. The coronal, middle and apical thirds sections showed dye penetration of 1.90 ± 1.4 mm2, 1.16 ± 0.9 mm2 and 0.43 ± 0.4 mm2, respectively.
Conclusions: TG-Cleanser showed superior efficacy in removing smear layer compared to SmearOFF at coronal, middle and apical root sections.
Methods: It was an in-vitro experimental study. Sixty teeth were randomly allocated into two experimental groups. Teeth in each group were standardized to 16mm form root apex and crowns were removed. After chemo-mechanical preparation of roots with ProTaper Next rotary file system, till up to X3 file to standardize root canal flare, roots were divided into two experimental groups. After irrigation with final designated irrigant, 1% methylene blue dye was filled and dried with paper points from root canal. Each root was sectioned horizontally at 2, 5 and 8mm, representing apical, middle and coronal sections, respectively and examined under stereomicroscope (magnification X25.6). Area of dye penetration was measured using ImageJ software. Area of dye penetration in mm2 was recorded as the irrigant efficacy. Factorial design ANOVA was applied to compare area of methylene blue dye penetration between two groups (SmearOFF TM versus TG-Cleanser) at coronal, middle and apical root sections. A p-value of < 0.05 was taken as statistically significant.
Results: Teeth in TG-Cleanser showed a mean dye penetration of 1.69 ±1.3 mm2, whereas, SmearOFF showed a mean dye penetration 0.63 ±0.7 mm2. The coronal, middle and apical thirds sections showed dye penetration of 1.90 ± 1.4 mm2, 1.16 ± 0.9 mm2 and 0.43 ± 0.4 mm2, respectively.
Conclusions: TG-Cleanser showed superior efficacy in removing smear layer compared to SmearOFF at coronal, middle and apical root sections.