IADR Abstract Archives
Preparation and in-vitro characterizations of novel Bioactive Glass Fibres
Objectives: In recent times, bioceramics are the material which not only biocompatible but also initiate bioactive process when placed inside the human body. Glass fibers based biomedical materials have gained interest for clinical application due to their better mechanical and physical properties. The aim of this study was to make E-glass fiber bioactive by a novel technique using microwave irradiation.
Methods: Industrial E-glass fibers were initially cut into small pieces and surface activated with heat and acid treatment. The calcium and phosphorous precursors were used to develop nano-apatite structure. The surface activated E-glass fibers in two ratios i.e. 30% and 50% wt/wt were added in calcium/phosphorous precursors, later the solution was irradiated in microwave to get nano-hydroxyapatite (nHA) particles on E-glass fibers (nHA/E30 and nHA/E50). The nHA/E0 was used as control. The synthesized material was characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis/Differential Scanning Calorimetry (TGA/DSC), Scanning electron Microscopy (SEM) and in vitro biocompatibility testing has been done on rat mesenchymal stem cells (MSC) for 3 and 7 day and cellular response was observed by MTT Assay. Statistical analysis was performed by One way ANOVA by using SPSS 22.
Results: The FTIR and XRD confirmed the presence of nHA on E-glass fiber, where, characteristics peaks were appeared. TGA showed enhanced thermal stability of the prepared sample due to E-glass fibers. Scanning Electron Microscopic (SEM) images confirmed the homogenous adhesion of nHA spherical particles all over the fibers. Cell viability with mesenchymal stem cells showed growth, proliferation, and adhesion which was enhanced by the presence of surface grafted E-glass. The % viability of nHA/E30 and nHA/E50 was significantly higher (p ≤ 0.05) than nHA/E0
Conclusions: It appeared that this novel bioactive eglass fibers might potentially be developed into a clinically attractive materials to be used for different dental and biomedical applications.
Methods: Industrial E-glass fibers were initially cut into small pieces and surface activated with heat and acid treatment. The calcium and phosphorous precursors were used to develop nano-apatite structure. The surface activated E-glass fibers in two ratios i.e. 30% and 50% wt/wt were added in calcium/phosphorous precursors, later the solution was irradiated in microwave to get nano-hydroxyapatite (nHA) particles on E-glass fibers (nHA/E30 and nHA/E50). The nHA/E0 was used as control. The synthesized material was characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis/Differential Scanning Calorimetry (TGA/DSC), Scanning electron Microscopy (SEM) and in vitro biocompatibility testing has been done on rat mesenchymal stem cells (MSC) for 3 and 7 day and cellular response was observed by MTT Assay. Statistical analysis was performed by One way ANOVA by using SPSS 22.
Results: The FTIR and XRD confirmed the presence of nHA on E-glass fiber, where, characteristics peaks were appeared. TGA showed enhanced thermal stability of the prepared sample due to E-glass fibers. Scanning Electron Microscopic (SEM) images confirmed the homogenous adhesion of nHA spherical particles all over the fibers. Cell viability with mesenchymal stem cells showed growth, proliferation, and adhesion which was enhanced by the presence of surface grafted E-glass. The % viability of nHA/E30 and nHA/E50 was significantly higher (p ≤ 0.05) than nHA/E0
Conclusions: It appeared that this novel bioactive eglass fibers might potentially be developed into a clinically attractive materials to be used for different dental and biomedical applications.