IADR Abstract Archives
Evaluation of Dentine Regeneration by Injectable Bioactive Glass: An in vitro study
Objectives: Dentine loss is a major concern due to caries. Therefore objective was to evaluate dentine regeneration potential of novel injectable bioactive glass material in bovine teeth to act as lining material under tooth restorations.
Methods: Injectable bioactive glass with novel composition was synthesized and injected in dentin portion of extracted bovine teeth while coronal portion was covered with composite. The samples were kept in deionized water and artificial saliva at 37°C and retrieved at day 7, 21, 30, 90 and 180. The area covered by newly formed apatite was calculated using images from SEM and ImageJ. Elemental analysis was carried out by EDS.
Results: Day 7, the mean covered area by newly formed apatite layer calculated for samples in artificial saliva was 6.5% and for deionized water was 11.50%. On day 21, the samples retrieved showed 39.8% and 25.4% area covered by newly formed apatite layer in artificial saliva and deionized water, respectively. In deionized water EDS showed 0.12%-wt silica while in artificial saliva, it was 0.06%-wt silica. On 30th day, the mean covered area was found to be 60.40% and 58.20% in artificial saliva and deionized water, respectively. Area analyses of samples in artificial saliva were found to have 4.47%-wt Si. Day 90 samples showed 82.20% and 58.40% area covered by newly formed apatite in artificial saliva and deionized water. During elemental area analysis, 3.85%-wt. and 10.40%-wt. Si was found in artificial saliva and deionized water respectively. Day 180 samples showed 96.23% and 87.05% in artificial saliva and deionized water, respectively. EDS area analysis reveals 18.65%-wt. Si in artificial saliva and 5.44%-wt. in deionized water.
Conclusions: These findings indicate that the injectable bioactive glass is capable of forming a new apatite layer. This layer increased with each interval of time. The presence of silica in newly formed apatite indicates bioactive glass is the source of new apatite.
Methods: Injectable bioactive glass with novel composition was synthesized and injected in dentin portion of extracted bovine teeth while coronal portion was covered with composite. The samples were kept in deionized water and artificial saliva at 37°C and retrieved at day 7, 21, 30, 90 and 180. The area covered by newly formed apatite was calculated using images from SEM and ImageJ. Elemental analysis was carried out by EDS.
Results: Day 7, the mean covered area by newly formed apatite layer calculated for samples in artificial saliva was 6.5% and for deionized water was 11.50%. On day 21, the samples retrieved showed 39.8% and 25.4% area covered by newly formed apatite layer in artificial saliva and deionized water, respectively. In deionized water EDS showed 0.12%-wt silica while in artificial saliva, it was 0.06%-wt silica. On 30th day, the mean covered area was found to be 60.40% and 58.20% in artificial saliva and deionized water, respectively. Area analyses of samples in artificial saliva were found to have 4.47%-wt Si. Day 90 samples showed 82.20% and 58.40% area covered by newly formed apatite in artificial saliva and deionized water. During elemental area analysis, 3.85%-wt. and 10.40%-wt. Si was found in artificial saliva and deionized water respectively. Day 180 samples showed 96.23% and 87.05% in artificial saliva and deionized water, respectively. EDS area analysis reveals 18.65%-wt. Si in artificial saliva and 5.44%-wt. in deionized water.
Conclusions: These findings indicate that the injectable bioactive glass is capable of forming a new apatite layer. This layer increased with each interval of time. The presence of silica in newly formed apatite indicates bioactive glass is the source of new apatite.