IADR Abstract Archives
Activation of STAT3 on CD45+ cells in Human Gingival Tissues
Objectives: Periodontitis is a chronic inflammatory disease in which the locally deployed immune response against a dysbiotic subgingival microbiome results in alveolar bone resorption and tooth loss. The transcription factorsignal transducer and activator of transcription 3 (STAT3) integrates and transduces the signaling of multiple pro-inflammatory cytokines associated with periodontitis. STAT3 is also crucial for Th17 cell differentiation, a critical CD4+ T cell subset in periodontitis immunopathogenesis. Despite the importance of this protein, there is a gap in our knowledge regarding which cell type activates STAT3 (pSAT3) in human periodontal tissues. Hence, this study aimed to evaluate the STAT3 activation on hematopoietic (CD45+) cells in gingival tissues from healthy and periodontitis subjects.
Methods: After clinical evaluation, diagnosis, selection, and consent, a standardized gingival tissue sample was obtained from each volunteer. Gingival tissues were fixed, included in paraffin, and activation of STAT3 and immune cells was evaluated via immunofluorescence using an anti-pSTAT3 and anti-CD45 antibody. Immuno-positive cells were counted in 18 tissues (9 healthy and 9 periodontitis) using the CellProfiler™ software. Data are shown in mean ± SEM. P-values of <0.05 were considered statistically significant.
Results: Eighteen tissues were analyzed. Total percentages and numbers of CD45 and pSTAT3 immune-positive cells were significantly higher in the periodontitis tissues than compared with the healthy gingival tissues (24,9% ± 5,6% of CD45+ and 40,9% ± 10,2% of pSTAT3+). Furthemore pSTAT3-CD45 immune-positive cells were significantly higher of in the periodontitis tissues (33% ± 10%).
Conclusions: Immune cells and STAT3 transcription factor are more activated in gingival tissues obtained from subjects with periodontitis. Furthermore, there were significantly more pSTAT3-CD45 immune-positive cells detected from periodontitis tissue.
Methods: After clinical evaluation, diagnosis, selection, and consent, a standardized gingival tissue sample was obtained from each volunteer. Gingival tissues were fixed, included in paraffin, and activation of STAT3 and immune cells was evaluated via immunofluorescence using an anti-pSTAT3 and anti-CD45 antibody. Immuno-positive cells were counted in 18 tissues (9 healthy and 9 periodontitis) using the CellProfiler™ software. Data are shown in mean ± SEM. P-values of <0.05 were considered statistically significant.
Results: Eighteen tissues were analyzed. Total percentages and numbers of CD45 and pSTAT3 immune-positive cells were significantly higher in the periodontitis tissues than compared with the healthy gingival tissues (24,9% ± 5,6% of CD45+ and 40,9% ± 10,2% of pSTAT3+). Furthemore pSTAT3-CD45 immune-positive cells were significantly higher of in the periodontitis tissues (33% ± 10%).
Conclusions: Immune cells and STAT3 transcription factor are more activated in gingival tissues obtained from subjects with periodontitis. Furthermore, there were significantly more pSTAT3-CD45 immune-positive cells detected from periodontitis tissue.