IADR Abstract Archives
A Novel Bifunctional Nanocarrier with Osteogenic and Immune-modulatory Potential
Objectives: Chronic inflammatory response againt pathogenic subgingival biofilm in periodontitis leads to alveolar bone resorption and consequently tooth loss. A material with both anti-inflammatory and osteogenic properties would be ideal to treat bone defects left by periodontitis. Threfore, the aim of this investigation is to synthesize and characterize the combination of osteogenic bioactive glass nanoparticles loaded with IL-4, an anti-inflammatory interleukin, for the potential treatment of periodontitis.
Methods: Mesoporous bioactive glass nanospheres (nMBG) were synthesized by an hydrothermal method, using cetyltrimethylammonium bromide (CTABr) and polyvinylpyrrolidone (PVP) as cotemplates.Then, a saturated IL-4 solution was loaded in nMBG, loading and release of IL-4 was tested by UV-Spectrophotometry. nMBG/IL-4 characterization was done with SEM-TEM, EDX and FTIR-ATR analyses. Cell viability was tested by MTS and Trypan Blue Assays in gingival keratinocytes. To test the osteogenic potential, RT-qPCR for ALP and RUNX2, and Alizarin Red tests were performed in nMBG/IL-4-stimulated dental pulp stem cells. For immune-modulatory potential, RT-qPCR for TNF-α, IL-6, IL-8 and IL-10 was performed in nMBG/IL-4-stimulated keratinocytes.
Results: nMBG were spherical and homogenous (90nm - diameter), with highly ordered pores and with a surface area of 443m2/g.IL-4 was succesfully conjugated with nMBG, presenting characteristical peaks of silanol groups of nMBG and additional protein peaks (IL-4). nMBG/IL-4 did not reduce cell viability. nMBG/IL-4 significantly increased ALP expression and the presence of calcified deposits after applying it to stem cells. It is expected that, nMBG/IL-4 will decrease TNF-α, IL-6 and IL-8 expression and increase IL-10 expression in keratinocytes.
Conclusions: The combination of IL-4 with bioactive glass has potential osteogenic and immune-modulatory properties and could have a therapeutical use in oral osteo-immune pathologies like periodonitits, periimplantitis or osteoarthritis.
Methods: Mesoporous bioactive glass nanospheres (nMBG) were synthesized by an hydrothermal method, using cetyltrimethylammonium bromide (CTABr) and polyvinylpyrrolidone (PVP) as cotemplates.Then, a saturated IL-4 solution was loaded in nMBG, loading and release of IL-4 was tested by UV-Spectrophotometry. nMBG/IL-4 characterization was done with SEM-TEM, EDX and FTIR-ATR analyses. Cell viability was tested by MTS and Trypan Blue Assays in gingival keratinocytes. To test the osteogenic potential, RT-qPCR for ALP and RUNX2, and Alizarin Red tests were performed in nMBG/IL-4-stimulated dental pulp stem cells. For immune-modulatory potential, RT-qPCR for TNF-α, IL-6, IL-8 and IL-10 was performed in nMBG/IL-4-stimulated keratinocytes.
Results: nMBG were spherical and homogenous (90nm - diameter), with highly ordered pores and with a surface area of 443m2/g.IL-4 was succesfully conjugated with nMBG, presenting characteristical peaks of silanol groups of nMBG and additional protein peaks (IL-4). nMBG/IL-4 did not reduce cell viability. nMBG/IL-4 significantly increased ALP expression and the presence of calcified deposits after applying it to stem cells. It is expected that, nMBG/IL-4 will decrease TNF-α, IL-6 and IL-8 expression and increase IL-10 expression in keratinocytes.
Conclusions: The combination of IL-4 with bioactive glass has potential osteogenic and immune-modulatory properties and could have a therapeutical use in oral osteo-immune pathologies like periodonitits, periimplantitis or osteoarthritis.